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Coleus blumei viroid 6: a new tentative member of the genus Coleviroid derived from natural genome shuffling

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Abstract

Coleus blumei can be infected by several viroids of the genus Coleviroid. One year after detecting a mixed infection of coleus blumei viroid 1 (CbVd-1) and 5 (CbVd-5) in coleus seedlings inoculated with these two viroids, we found an additional viroid-like RNA. Sequence analysis revealed a viroid of 342 nucleotides that contains the central conserved region of coleviroids and is a chimera of the left half of CbVd-3 and the right half of CbVd-5. This new viroid, tentatively referred to as coleus blumei viroid 6 (CbVd-6), appears to have arisen from a natural recombination event or genome shuffling.

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Acknowledgments

This work was supported by grants from the National Basic Research and Development Program of China (973 Program) (No. 2009CB119200 and No. 2006 CB100203), the National Natural Science Foundation of China (No.30771403),the National Key Technologies Research and Development Program (No. 2006 BAD08A14),the National High Technology Research and Development Program (863 Program) (No.2006AA10Z432), the Beijing Natural Science Foundation of China (No. 6072022), and the Opening Project of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences. This work was also supported by NSFC-JSPS Joint Research Project between China and Japan (30811140157).

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Authors and Affiliations

  1. State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road No2, Haidian District, 100193, Beijing, People’s Republic of China

    Wan-Ying Hou & Shi-Fang Li

  2. Department of Plant Pathology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan

    Teruo Sano

  3. Institute of Plant Virology, Fujian Agriculture and Forestry University, Jinshan, 350002, Fuzhou, Fujian, People’s Republic of China

    Zu-Jian Wu & Dong-Mei Jiang

Authors
  1. Wan-Ying Hou
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  2. Shi-Fang Li
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  3. Zu-Jian Wu
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  4. Dong-Mei Jiang
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  5. Teruo Sano
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Corresponding authors

Correspondence to Shi-Fang Li or Teruo Sano.

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S. Li and T. Sano contributed equally to this work.

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Hou, WY., Li, SF., Wu, ZJ. et al. Coleus blumei viroid 6: a new tentative member of the genus Coleviroid derived from natural genome shuffling. Arch Virol 154, 993–997 (2009). https://doi.org/10.1007/s00705-009-0388-7

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  • DOI: https://doi.org/10.1007/s00705-009-0388-7

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