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Variations in the H/ACA box sequence of viral telomerase RNA of isolates of CVI988 Rispens vaccine

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Abstract

The use of the complete DNA sequence for the Marek’s disease virus (MDV) serotype 1 vaccine strain CVI988 Rispens in comparative genomic studies with virulent strains of MDV has revealed the presence of a number of insertions, deletions and single-nucleotide polymorphisms. In this study, we investigated a SNP in the H/ACA box of the viral RNA subunit of telomerase (vTR). We sequenced vTR from four different batches of CVI988 vaccine originating from a single commercial company. The A-to-G mutation defining the SNP in the H/ACA box of CVI988 vTR was present in only some of the batches. Thus, although this mutation affects CVI988 vTR function, it is not shared by all CVI988 isolates and may be a stochastic rather than causative event in CVI988 attenuation.

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Acknowledgments

Manel Debba-Pavard was a recipient of grants from the Algerian Education and Research Ministry. We would like to thank Sylvie Laurent and Sylvie Remy for comments on the manuscript. This work was supported by grants from the Ligue Nationale contre le Cancer-Comité du Cher.

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Authors and Affiliations

  1. Equipe Télomérase et Lymphome Viro-Induit Unité IASP1282, INRA, 37380, Nouzilly, France

    Manel Debba-Pavard, Ginette Dambrine & Denis Rasschaert

  2. Fort Dodge Animal Health, Huizerstraatweg 117, 1411 GM, Naarden, The Netherlands

    Hervé Le Galludec

  3. Université François-Rabelais Sciences et Techniques, Parc de Grandmont, 37200, Tours, France

    Denis Rasschaert

Authors
  1. Manel Debba-Pavard
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  2. Hervé Le Galludec
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  3. Ginette Dambrine
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  4. Denis Rasschaert
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Correspondence to Denis Rasschaert.

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Debba-Pavard, M., Le Galludec, H., Dambrine, G. et al. Variations in the H/ACA box sequence of viral telomerase RNA of isolates of CVI988 Rispens vaccine. Arch Virol 153, 1563–1568 (2008). https://doi.org/10.1007/s00705-008-0152-4

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  • DOI: https://doi.org/10.1007/s00705-008-0152-4

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