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Mapping the 5′ ends of banana bunchy top virus gene transcripts

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Summary.

Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3′ rapid amplification of cDNA ends (3′ RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5′ cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.

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Authors and Affiliations

  1. Centre for Tropical Crops and Biocommodities, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia

    V. A. Herrera-Valencia, B. Dugdale, R. M. Harding & J. L. Dale

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  1. V. A. Herrera-Valencia
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  2. B. Dugdale
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  3. R. M. Harding
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  4. J. L. Dale
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Herrera-Valencia, V., Dugdale, B., Harding, R. et al. Mapping the 5′ ends of banana bunchy top virus gene transcripts. Arch Virol 152, 615–620 (2007). https://doi.org/10.1007/s00705-006-0889-6

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  • DOI: https://doi.org/10.1007/s00705-006-0889-6

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