Summary.
The recent observation of the frequent occurrence of natural recombinant Plum pox virus (PPV) isolates has led to the identification of a distinct PPV subgroup, named PPV-Rec. The diversity, origin and geographical spread of the recombinant PPV isolates belonging to this subgroup remain, however, relatively poorly known. In an effort to further our understanding of these isolates, eight PPV isolates from Serbia, the country from which the first such recombinant (PPV-o6) originated, were characterized. Depending on the genomic region targeted by different typing assays, seven of the eight isolates tested presented discrepancies in their typing behavior. Sequence analysis of the (Cter)NIb-(Nter)CP region confirmed the recombinant nature of these seven isolates which all presented an identical recombination breakpoint identical to previously characterized PPV-Rec isolates. Biological indexing and immunoblot analysis provided indications that asymptomatic infection of the GF305 peach indicator and migration of the coat protein as a double-band in immunoblots may represent conserved and discriminating properties of PPV-Rec isolates. The genetic diversity of PPV-Rec isolates from former Yugoslavia (Serbia, Bosnia and Herzegovina) was estimated to be twice as large as that of the PPV-Rec isolates obtained from all other countries to date (Albania, Bulgaria, Czech republic, Germany, Hungary and Slovakia). These last results are consistent with the hypothesis that former Yugoslavia is the center of dispersion of PPV-Rec. Taken together, the results presented here provide further evidence for the wide distribution and temporal genetic stability of these natural PPV recombinant isolates and provide for the first time a possible scenario for their dispersion throughout central and eastern Europe.
Similar content being viewed by others
References
M Bousalem T Candresse L Quiot-Douine JB Quiot (1994) ArticleTitleComparison of three methods for assessing plum pox virus variability: further evidence for the existence of two major groups of isolates. J Phytopathol 142 163–172
M Bousalem EJ Douzery D Fargette (2000) ArticleTitleHigh genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of Yam mosaic virus: a contribution to understanding potyvirus evolution. J Gen Virol 81 243–255 Occurrence Handle10640564
T Candresse M Cambra S Dallot M Lanneau M Asensio MT Gorris F Revers G Macquaire A Olmos D Boscia JB Quiot J Dunez (1998) ArticleTitleComparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the M and D serotypes of plum pox potyvirus. Phytopathology 88 198–204
MT Cervera JL Riechmann MT Martin JA Garcia (1993) ArticleTitle3′ terminal sequence of the plum pox virus PS and o6 isolates: evidence for RNA recombination within the potyvirus group. J Gen Virol 74 329–334 Occurrence Handle8445362
S Dallot G Labonne L Quiot-Douine M Boeglin T Candresse JB Quiot (1998) ArticleTitlePeculiar plum pox virus D-populations are epidemics in peach trees. Acta Hortic 472 355–365
VD Damsteegt HE Waterworth GI Mink WE Howell L Levy (1997) ArticleTitlePrunus tomentosa as a diagnostic host for detection of Plum pox virus and other Prunus viruses. Plant Dis 81 329–332
C Desbiez H Lecoq (2004) ArticleTitleThe nucleotide sequence of Watermelon mosaic virus (WMV, Potyvirus) reveals interspecific recombination between two related potyviruses in the 5′ part of the genome. Arch Virol 149 1619–1632 Occurrence Handle10.1007/s00705-004-0340-9 Occurrence Handle15290385
F Garcia-Arenal A Fraile JM Malpica (2001) ArticleTitleVariability and genetic structure of plant virus populations. Annu Rev Phytopathol 39 157–186 Occurrence Handle10.1146/annurev.phyto.39.1.157 Occurrence Handle11701863
M Glasa V Marie-Jeanne B Moury O Kúdela JB Quiot (2002a) ArticleTitleMolecular variability of the P3-6K1 genomic region among geographically and biologically distinct isolates of Plum pox virus. Arch Virol 147 563–575 Occurrence Handle10.1007/s007050200006
M Glasa V Marie-Jeanne G Labonne Z Šubr O Kúdela JB Quiot (2002b) ArticleTitleA natural population of recombinant Plum pox virus is stable and competitive under field conditions. Eur J Plant Pathol 108 843–853 Occurrence Handle10.1023/A:1021294221878
M Glasa L Palkovics P Komínek G Labonne S Pittnerová O Kúdela T Candresse Z Šubr (2004) ArticleTitleGeographically and temporally distant natural recombinant isolates of Plum pox virus are genetically very similar and form a unique PPV subgroup. J Gen Virol 85 2671–2681 Occurrence Handle10.1099/vir.0.80206-0 Occurrence Handle15302961
M Glasa T Candresse (2005) ArticleTitlePartial sequence analysis of an atypical Turkish isolate provides further information on the evolutionary history of Plum pox virus (PPV). Virus Res 108 199–206 Occurrence Handle10.1016/j.virusres.2004.09.009 Occurrence Handle15681071
D James A Varga D Thompson S Hayes (2003) ArticleTitleDetection of a new and unusual isolate of plum pox potyvirus in plum (Prunus domestica). Plant Dis 87 1119–1124
S Kumar K Tamura BJ Ingrid M Nei (2001) ArticleTitleMEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17 1244–1245 Occurrence Handle10.1093/bioinformatics/17.12.1244 Occurrence Handle11751241
Matic S, Al-Rwahnih M, Myrta A (2004) Typing of Plum pox virus isolates in Bosnia and Herzegovina. In: Eur Meeting ’04 on Plum pox, Rogów, Poland, Abstr. p 16
F Moonan J Molina TE Mirkov (2000) ArticleTitleSugarcane yellow leaf virus: an emerging virus that has evolved by recombination between Luteoviral and Poleroviral ancestors. Virology 269 156–171 Occurrence Handle10.1006/viro.1999.0162 Occurrence Handle10725208
Nemchinov L, Crescenzi A, Hadidi A, Piazzolla P, Verderevskaya T (1998) Present status of the new cherry subgroup of Plum pox virus (PPV-C). In: Hadidi A, Khetarpal RK, Koganezawa H (eds) Plant virus disease control, pp 629–638. APS Press St Paul, Minnesota
DR Page (1996) ArticleTitleTreeView: an application to display phylogenetic trees on personal computer. Comp Appl Biosci 12 357–358 Occurrence Handle8902363
JL Riechmann S Lain JA Garcia (1992) ArticleTitleHighlights and prospects of potyvirus molecular biology. J Gen Virol 73 1–16 Occurrence Handle1730931
J Rozas JC Sánchez-DelBarrio X Messeguer R Rozas (2003) ArticleTitleDnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19 2496–2497 Occurrence Handle10.1093/bioinformatics/btg359 Occurrence Handle14668244
Z Šubr M Glasa (1999) ArticleTitlePlum pox virus capsid protein mobility in SDS-polyacrylamide gel electrophoresis. Acta Virol 43 259–262 Occurrence Handle10749373
Z Šubr S Pittnerová M Glasa (2004) ArticleTitleA simplified RT-PCR-based detection of recombinant Plum pox virus isolates. Acta Virol 48 173–176 Occurrence Handle15595211
JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24 4876–4882 Occurrence Handle10.1093/nar/25.24.4876
K Tomimura AJ Gibbs CE Jenner JA Walsh K Ohshima (2003) ArticleTitleThe phylogeny of Turnip mosaic virus; comparisons of 38 genomic sequences reveal a Eurasian origin and a recent ‘emergence’ in east Asia. Mol Ecol 12 2099–2111 Occurrence Handle10.1046/j.1365-294X.2003.01881.x Occurrence Handle12859632
GF Weiller (1998) ArticleTitlePhylogenetic profiles: a graphical method for detecting genetic recombinations in homologous sequences. Mol Biol Evol 15 326–335 Occurrence Handle9501499
T Wetzel T Candresse M Ravelonandro J Dunez (1991a) ArticleTitleA polymerase chain reaction assay adapted to plum pox potyvirus detection. J Virol Methods 33 355–365 Occurrence Handle10.1016/0166-0934(91)90035-X
T Wetzel T Candresse M Ravelonandro RP Delbos H Mazyad AE Aboul-Ata J Dunez (1991b) ArticleTitleNucleotide sequence of the 3′-terminal region of the RNA of the El Amar strain of plum pox potyvirus. J Gen Virol 72 1741–1746
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Glasa, M., Paunovic, S., Jevremovic, D. et al. Analysis of recombinant Plum pox virus (PPV) isolates from Serbia confirms genetic homogeneity and supports a regional origin for the PPV-Rec subgroup. Arch Virol 150, 2051–2060 (2005). https://doi.org/10.1007/s00705-005-0548-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-005-0548-3