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Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode modified with a dendrimer/silver nanoparticle nanocomposite

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Abstract

The authors describe a highly sensitive method for the aptamer (Apt) based impedimetric determination of cocaine. The surface of a screen-printed electrode (SPE) was modified with a nanocomposite of dendrimer and silver nanoparticles (AgNPs). The cocaine-binding Apt was attached to a dendrimer/AgNP/SPE surface, forming a sensitive layer for the determination of cocaine. The incubation with the analyte resulted in the formation of a cocaine/Apt complex on the electrode surface. As a consequence, folding and conformational change in the aptamer structure was induced, this resulting in a change in the impedimetric signal. The aptaassay exhibits highly efficient sensing characteristics with a good linearity of 1 fmol L−1 to 100 nmol L−1 (with two linear ranges) and a limit of detection (LOD) of 333 amol L−1. Its excellent specificity and high sensitivity suggest that this kind of aptamer-based assay may be applied to detect other targets in this field.

Designing of an aptaassay via immobilization of a functionalized aptamer with silver nanoparticle (AgNPs-Apt) on the modified screen-printed electrode (SPE) with dendrimer/silver nanoparticle nanocomposite (Den-AgNPs) for impedimetric detection of cocaine.

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References

  1. Hart JP, Crew A, Crouch E, Honeychurch KC, Pemberton RM (2004) Some recent designs and developments of screen-printed carbon electrochemical sensor/biosensors for biomedical, environmental and industrial analyses (review). Anal Lett 37:789–830

    Article  CAS  Google Scholar 

  2. Taleat Z, Khoshroo A, Mazloum-Ardakani M (2014) Screen-printed electrodes for biosensing: a review (2008–2013). Microchim Acta 181(9):865–891

    Article  CAS  Google Scholar 

  3. Bonanni A, Esplandiu MJ, Valle M (2009) Impedimetric genosensors employing COOH-modified carbon nanotube screenprinted electrodes. Biosens Bioelectron 24:2885–2891

    Article  CAS  Google Scholar 

  4. Zamaleeva AI, Sharipova IR, Shamagsumova RV, Ivanov AN, Evtugyn GA, Ishmuchametova DG, Fakhrullin RF (2011) A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes. Anal Methods 3:509–513

    Article  CAS  Google Scholar 

  5. Liu J, Yuan X, Gao Q, Honglan Q, Chengxiao Z (2012) Ultrasensitive DNA detection based on coulometric measurement of enzymatic silver deposition on gold nanoparticle-modified screen-printed carbon electrode. Sensors Actuators B Chem 162:384–390

    Article  CAS  Google Scholar 

  6. Lee SJ, Lytton-Jean AK, Hurst SJ, Mirkin CA (2007) Silver nanoparticle−oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties. Nano Lett 7:2112–2115

    Article  CAS  Google Scholar 

  7. Lee SC, Parthasarathy R, Duffin TDY, Botwin K, Zobel J, Beck T, Lange G, Kunneman D, Janseen R, Rowold E, Voliva CF (2001) Recognition properties ofantibodies to PAMAM dendrimers and their use in immune detection of den-drimers. Biomed Microdevices 3:53–59

    Article  CAS  Google Scholar 

  8. Yang W, Cheng Y, Xu T, Wang X, Wen LG (2009) Targeting cancer cells withbiotin–dendrimer conjugates. Eur J Med Chem 44:862–868

    Article  CAS  Google Scholar 

  9. Khopade AJ, Caruso F, Tripathi P, Nagaich S, Jain NK (2002) Effect of dendrimer onentrapment and release of bioactive from liposomes. Int J Pharm 232:157–162

    Article  CAS  Google Scholar 

  10. Álvarez-Martos I, Shahdost-fard F, Ferapontova EE (2017) Wiring of heme enzymes by methylene-blue labeled dendrimers. Electrochim Acta 249:206–215

    Article  Google Scholar 

  11. Iliuk AB, Hu L, Tao WA (2011) Aptamer in bioanalytical applications. Anal Chem 83:4440–4452

    Article  CAS  Google Scholar 

  12. Center for Behavioral Health Statistics and Quality (CBHSQ) (2015) Behavioral Health Trends in the United States: Results from the 2014 National Survey on Drug Use and Health. Rockville, MD: Substance Abuse and Mental Health Services Administration. HHS Publication No. SMA 15–4927, NSDUH Series H-50

  13. Krutyakov YA, Kudrinskiy AA, Olenin AY, Lisichkin GV (2008) Synthesis and properties of silver nanoparticles: advances and prospects. Russ Chem Rev 77:233–257

    Article  CAS  Google Scholar 

  14. Roushani M, Shahdost-fard F (2015) Novel ultrasensitive aptasensor based on silver nanoparticles measured via enhanced voltammetric response of electrochemical reduction of riboflavin as redox probe for cocaine detection. Sensors Actuators B Chem 207:764–771

    Article  CAS  Google Scholar 

  15. Morzyk-Ociepa B, Michalsk D (2003) Vibrational spectra of 1-methyluracilate complex with silver (I) and theoretical studies of the 1-MeU anion. Spectrochim Acta Part A 59:1247–1254

    Article  Google Scholar 

  16. Yao Z, Yang X, Wu F, Wu W, Wu F (2016) Synthesis of differently sized silver nanoparticles on a screen-printed electrode sensitized with a nanocomposites consisting of reduced graphene oxide and cerium (IV) oxide for nonenzymatic sensing of hydrogen peroxide. Microchim Acta 183:2799–2806

    Article  CAS  Google Scholar 

  17. Castillo G, Spinella K, Poturnayova A, Snejdarkova M, Mosiello L, Hianik T (2015) Detection of aflatoxin B1 by aptamer-based biosensor using PAMAM dendrimers as immobilization platform. Food Control 52:9–18

    Article  CAS  Google Scholar 

  18. Roushani M, Shahdost-fard F (2015) Fabrication of an ultrasensitive ibuprofen nanoaptasensor based on covalent attachment of aptamer to electrochemically deposited gold-nanoparticles on glassy carbon electrode. Talanta 144:510–516

    Article  CAS  Google Scholar 

  19. Shahdost-fard F, Roushani M (2016) Conformation switching of an aptamer based on cocaine enhancement on a surface of modified GCE. Talanta 154:7–14

    Article  CAS  Google Scholar 

  20. Roushani M, Shahdost-fard F (2016) Fabrication of an electrochemical nanoaptasensor based on AuNPs for ultrasensitive determination of cocaine in serum sample. Mater Sci Eng C 61:599–607

    Article  CAS  Google Scholar 

  21. Roushani M, Shahdost-fard F (2016) An aptasensor for voltammetric and impedimetric determination of cocaine based on a glassy carbon electrode modified with platinum nanoparticles and using rutin as a redox probe. Microchim Acta 183(1):185–193

    Article  CAS  Google Scholar 

  22. Jiang B, Wang M, Chen Y, Xie J, Xiang Y (2012) Highly sensitive electrochemical detection of cocaineon graphene/AuNP modified electrod via catalytic redox- recycling amplification. Biosens Bioelectron 32:305–308

    Article  CAS  Google Scholar 

  23. Du Y, Chen C, Yin J, Li B, Zhou M, Dong S, Wang E (2010) Solid-state probe based electrochemical aptasensor for cocaine: a potentially convenient, sensitive, repeatable, and integrated sensing platform for drugs. Anal Chem 82:1556–1563

    Article  CAS  Google Scholar 

  24. Zhang DW, Sun CJ, Zhang FT, Xu L, Zhou YL, Zhang XX (2012) An electrochemical aptasensor based on enzyme linked aptamer assay. Biosens Bioelectron 31:363–368

    Article  CAS  Google Scholar 

  25. He JL, Yang YF, Shen GL, Yu RQ (2011) Electrochemical aptameric sensor based on the Klenow fragment polymerase reaction for cocaine detection. Biosens Bioelectron 26:4222–4226

    Article  CAS  Google Scholar 

  26. Freeman R, Sharon E, Tel-Vered R, Willner I (2009) Supramolecular cocaine–aptamer complexes activate biocatalytic cascades. J Am Chem Soc 131:5028–5029

    Article  CAS  Google Scholar 

  27. Sheng Q, Liu R, Zhang S, Zheng J (2014) Ultrasensitive electrochemical cocaine biosensor based on reversible DNA nanostructure. Biosens Bioelectron 51:191–194

    Article  CAS  Google Scholar 

  28. Zhang DW, Zhang FT, Cui YR, Deng QP, Krause S, Zhou YL, Zhang XX (2012) A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy. Talanta 92:65–71

    Article  CAS  Google Scholar 

  29. Liu J, Lu Y (2006) Fast colorimetric sensing of adenosine and cocaine based on a general sensor design involving aptamers and nanoparticles. Angew Chem 118:96–100

    Article  Google Scholar 

  30. Stojanovic MN, Landry DW (2002) Aptamer-based colorimetric probe for cocaine. J Am Chem Soc 124:9678–9679

    Article  CAS  Google Scholar 

  31. Li Y, Ji X, Liu B (2011) Chemiluminescence aptasensor for cocaine based on double-functionalized gold nanoprobes and functionalized magnetic microbeads. Anal Bioanal Chem 401:213–219

    Article  CAS  Google Scholar 

  32. Shen B, Li J, Cheng W, Yan Y, Tang R, Li Y, Ju H, Ding S (2015) Electrochemical aptasensor for highly sensitive determination of cocaine using a supramolecular aptamer and rolling circle amplification. Microchim Acta 182(1):361–367

    Article  CAS  Google Scholar 

  33. Lesniak W, Bielinska AU, Sun K, Janczak KW, Shi X, Baker JR, Balogh LP (2005) Silver/dendrimer nanocomposites as biomarkers: fabrication, characterization, in vitro toxicity, and intracellular detection. Nano Lett 5:2123–2130

    Article  CAS  Google Scholar 

  34. Yu L, Shi Y, Zhao Z, Yin H, Wei Y, Liu J, Kang W, Jiang T, Wang A (2011) Ultrasmall silver nanoparticles supported on silica and their catalytic performances for carbon monoxide oxidation. Catal Commun 12:616–620

    Article  CAS  Google Scholar 

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Acknowledgements

The authors thank the Iran National Science Foundation (Grant no. 94808064) for their support. They would also like to thank the Ilam University Research Council and the Iranian Nanotechnology Initiative Council for their partial support.

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Authors and Affiliations

  1. Department of Chemistry, University of Ilam, Ilam, 69315-516, Iran

    Mahmoud Roushani & Faezeh Shahdost-fard

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  1. Mahmoud Roushani
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  2. Faezeh Shahdost-fard
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Correspondence to Mahmoud Roushani.

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Roushani, M., Shahdost-fard, F. Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode modified with a dendrimer/silver nanoparticle nanocomposite. Microchim Acta 185, 214 (2018). https://doi.org/10.1007/s00604-018-2709-6

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  • DOI: https://doi.org/10.1007/s00604-018-2709-6

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