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Layered vanadium(IV) disulfide nanosheets as a peroxidase-like nanozyme for colorimetric detection of glucose

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Abstract

The authors have discovered that vanadium disulfide (VS2) nanosheets, synthesized by a hydrothermal method, exert stable peroxidase-like activity. The catalytic activity, with H2O2 as a cosubstrate, follows Michaelis-Menten kinetics and varies with temperature, pH value and H2O2 concentration. Two-dimensional VS2 sheets acting as peroxidase (POx) mimics can replace horseradish peroxidase due to their availability, robustness, and reusability. The POx-like activity of VS2 sheets was exploited to design a colorimetric glucose assay by using 3,3′,5,5′-tetramethylbenzidine as a substrate and by working at an analytical wavelength of 652 nm. The assay covers the 5 to 250 μM glucose concentration range with a 1.5 μM detection limit. It was applied to the analysis of glucose in fruit juice. In our perception, the peroxidase-like nanozyme out of the family of transition metal dichalcogenides presented here has a wide scope in that it may stimulate promising biocatalytic applications in biotechnology and analytical chemistry.

Layered VS2 nanosheets were prepared via hydrothermal synthesis and are shown to exert superior peroxidase-mimicking activity. Using these POx nano-mimics, a sensitive colorimetric assay for glucose was developed and applied to fruit juice analysis. This work unlocks the access of VS2 to biocatalysis and bioassays.

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Acknowledgements

This research was financed by Grants from National Natural Science Foundation of China (No. 21675127), Fundamental Research Funds for the Northwest A&F University of China (2014YB093, 2452015257), and Development Project of Qinghai Key Laboratory (No. 2017-ZJ-Y10).

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Authors and Affiliations

  1. College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Lunjie Huang, Wenxin Zhu, Wentao Zhang, Kai Chen, Jing Wang, Rong Wang, Qingfeng Yang & Jianlong Wang

  2. Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China

    Na Hu & Yourui Suo

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  1. Lunjie Huang
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  2. Wenxin Zhu
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  3. Wentao Zhang
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  4. Kai Chen
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  5. Jing Wang
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  6. Rong Wang
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  7. Qingfeng Yang
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  8. Na Hu
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  10. Jianlong Wang
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Correspondence to Jianlong Wang.

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Huang, L., Zhu, W., Zhang, W. et al. Layered vanadium(IV) disulfide nanosheets as a peroxidase-like nanozyme for colorimetric detection of glucose. Microchim Acta 185, 7 (2018). https://doi.org/10.1007/s00604-017-2552-1

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