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The receptor locus for Escherichia coli F4ab/F4ac in the pig maps distal to the MUC4–LMLN region

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Abstract

Enterotoxigenic Escherichia coli (ETEC) with fimbriae of the F4 family are one of the major causes of diarrhea and death among neonatal and young piglets. Bacteria use the F4 fimbriae to adhere to specific receptors expressed on the surface of the enterocytes. F4 fimbriae exist in three different antigenic variants, F4ab, F4ac, and F4ad, of which F4ac is the most common. Resistance to ETEC F4ab/F4ac adhesion in pigs has been shown to be inherited as an autosomal recessive trait. In previous studies the ETEC F4ab/F4ac receptor locus (F4bcR) was mapped to the q41 region on pig chromosome 13. A polymorphism within an intron of the mucin 4 (MUC4) gene, which is one of the possible candidate genes located in this region, was shown earlier to cosegregate with the F4bcR alleles. Recently, we discovered a Large White boar from a Swiss experimental herd with a recombination between F4bcR and MUC4. A three–generation pedigree including 45 offspring was generated with the aim to use this recombination event to refine the localization of the F4bcR locus. All pigs were phenotyped using the microscopic adhesion test and genotyped for a total of 59 markers. The recombination event was mapped to a 220-kb region between a newly detected SNP in the leishmanolysin-like gene (LMLN g.15920) and SNP ALGA0072075. In this study the six SNPs ALGA0072075, ALGA0106330, MUC13-226, MUC13-813, DIA0000584, and MARC0006918 were in complete linkage disequilibrium with F4bcR. Based on this finding and earlier investigations, we suggest that the locus for F4bcR is located between the LMLN locus and microsatellite S0283.

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Acknowledgments

We thank Prof. Martien Groenen and his group (Animal Breeding and Genetics, Wageningen University, Wageningen, The Netherlands) for providing the SNP sequences and their positions in the Sscrofa9 assembly. We thank Michael Goe for editing the manuscript and the Genomic Diversity Center (ETH Zurich) for allowing us to use their infrastructure. The study was supported by the Swiss National Science Foundation (grant No. 3100A0-120255/1), by the ETH Zurich, and the SUISAG, Sempach (Dr. Andreas Hofer).

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Authors and Affiliations

  1. Institute of Plant, Animal and Agroecosystem Sciences, ETH Zurich, 8092, Zurich, Switzerland

    Antonio Rampoldi, Hans U. Bertschinger, David Joller, Peter Vögeli & Stefan Neuenschwander

  2. Basic Animal and Veterinary Sciences, University of Copenhagen, 1870 Frederiksberg, Copenhagen, Denmark

    Mette J. Jacobsen, Merete Fredholm & Claus B. Jørgensen

  3. Department of Farm Animals, University of Zurich, 8057, Zurich, Switzerland

    Esther Bürgi

  4. Department of Medical Biochemistry and Microbiology, Uppsala University, 751 23, Uppsala, Sweden

    Leif Andersson

  5. Division of Genetics and Genomics, The Roslin Institute and R(D)SVS, University of Edinburgh, Roslin, Midlothian, EH25 9PS, UK

    Alan L. Archibald

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  1. Antonio Rampoldi
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  2. Mette J. Jacobsen
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  3. Hans U. Bertschinger
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  4. David Joller
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  5. Esther Bürgi
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  6. Peter Vögeli
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  7. Leif Andersson
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  8. Alan L. Archibald
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  9. Merete Fredholm
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  10. Claus B. Jørgensen
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  11. Stefan Neuenschwander
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Corresponding author

Correspondence to Peter Vögeli.

Additional information

A. Rampoldi and M. J. Jacobsen contributed equally to this work.

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Rampoldi, A., Jacobsen, M.J., Bertschinger, H.U. et al. The receptor locus for Escherichia coli F4ab/F4ac in the pig maps distal to the MUC4–LMLN region. Mamm Genome 22, 122–129 (2011). https://doi.org/10.1007/s00335-010-9305-3

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  • DOI: https://doi.org/10.1007/s00335-010-9305-3

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