Abstract
The Dnmt3a and Dnmt3L genes are critical mediators of cytosine methylation during gametogenesis, with major actions noted at transposable elements and imprinted loci. The Dnmt3a–Dnmt3L complex was recently described to have preferential activity at CG dinucleotides located 8-10 bp apart. Because cytosine methylation is heterogeneously distributed in the genome, we tested whether this relative sequence preference explains the effects of mutation of the Dnmt3a and Dnmt3L genes using bioinformatic analysis. We found that the human and mouse genomes are significantly enriched in a CG dinucleotide periodicity of 2 bp, leading to an increased frequency of CGs spaced 8 bp apart that represent widespread targets for this protein complex. When we broke down the human and mouse genomes by annotation, we found that this significant 2-bp periodicity and increased 8-bp periodicity are maintained in Alu SINEs in both species. The 8-bp periodicity was mapped genome-wide, identifying enrichment at the promoters of both paternally and maternally methylated imprinted genes and at CG dinucleotide-enriched sequences. We conclude that CG dinucleotide periodicity helps to explain some but not all of the relative sequence specificity of mutations of Dnmt3a or Dnmt3L in the establishment of germline cytosine methylation patterns.
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Bock C, Paulsen M, Tierling S, Mikeska T, Lengauer T et al (2006) CpG island methylation in human lymphocytes is highly correlated with DNA sequence, repeats, and predicted DNA structure. PLoS Genet 2:e26
Bock C, Walter J, Paulsen M, Lengauer T (2007) CpG island mapping by epigenome prediction. PLoS Comput Biol 3:e110
Bourc’his D, Bestor TH (2004) Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L. Nature 431:96–99
Bourc’his D, Xu GL, Lin CS, Bollman B, Bestor TH (2001) Dnmt3L and the establishment of maternal genomic imprints. Science 294:2536–2539
Carroll ML, Roy-Engel AM, Nguyen SV, Salem AH, Vogel E et al (2001) Large-scale analysis of the Alu Ya5 and Yb8 subfamilies and their contribution to human genomic diversity. J Mol Biol 311:17–40
Ciccone DN, Su H, Hevi S, Gay F, Lei H et al (2009) KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 461:415–418
Clark SJ, Harrison J, Frommer M (1995) CpNpG methylation in mammalian cells. Nat Genet 10:20–27
Duncan BK, Miller JH (1980) Mutagenic deamination of cytosine residues in DNA. Nature 287:560–561
Edwards CA, Ferguson-Smith AC (2007) Mechanisms regulating imprinted genes in clusters. Curr Opin Cell Biol 19:281–289
Fazzari MJ, Greally JM (2004) Epigenomics: beyond CpG islands. Nat Rev Genet 5:446–455
Ferguson-Smith AC, Greally JM (2007) Epigenetics: perceptive enzymes. Nature 449:148–149
Fryxell KJ, Moon WJ (2005) CpG mutation rates in the human genome are highly dependent on local GC content. Mol Biol Evol 22:650–658
Gardiner-Garden M, Frommer M (1987) CpG islands in vertebrate genomes. J Mol Biol 196:261–282
Glass JL, Thompson RF, Khulan B, Figueroa ME, Olivier EN et al (2007) CG dinucleotide clustering is a species-specific property of the genome. Nucleic Acids Res 35:6798–6807
Greally JM (2002) Short interspersed transposable elements (SINEs) are excluded from imprinted regions in the human genome. Proc Natl Acad Sci USA 99:327–332
Hackenberg M, Previti C, Luque-Escamilla PL, Carpena P, Martinez-Aroza J et al (2006) CpGcluster: a distance-based algorithm for CpG-island detection. BMC Bioinformatics 7:446
Jia D, Jurkowska RZ, Zhang X, Jeltsch A, Cheng X (2007) Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation. Nature 449:248–251
Jurka J, Gentles AJ (2006) Origin and diversification of minisatellites derived from human Alu sequences. Gene 365:21–26
Kaneda M, Okano M, Hata K, Sado T, Tsujimoto N et al (2004) Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature 429:900–903
Kato Y, Kaneda M, Hata K, Kumaki K, Hisano M et al (2007) Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse. Hum Mol Genet 16:2272–2280
Ke X, Thomas NS, Robinson DO, Collins A (2002a) A novel approach for identifying candidate imprinted genes through sequence analysis of imprinted and control genes. Hum Genet 111:511–520
Ke X, Thomas SN, Robinson DO, Collins A (2002b) The distinguishing sequence characteristics of mouse imprinted genes. Mamm Genome 13:639–645
Khulan B, Thompson RF, Ye K, Fazzari MJ, Suzuki M et al (2006) Comparative isoschizomer profiling of cytosine methylation: the HELP assay. Genome Res 16:1046–1055
Klose RJ, Sarraf SA, Schmiedeberg L, McDermott SM, Stancheva I et al (2005) DNA binding selectivity of MeCP2 due to a requirement for A/T sequences adjacent to methyl-CpG. Mol Cell 19:667–678
Kriegs JO, Churakov G, Jurka J, Brosius J, Schmitz J (2007) Evolutionary history of 7SL RNA-derived SINEs in Supraprimates. Trends Genet 23:158–161
Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC et al (2001) Initial sequencing and analysis of the human genome. Nature 409:860–921
Lucifero D, La Salle S, Bourc’his D, Martel J, Bestor TH et al (2007) Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Dev Biol 7:36
Luedi PP, Hartemink AJ, Jirtle RL (2005) Genome-wide prediction of imprinted murine genes. Genome Res 15:875–884
Nagy PL, Cleary ML, Brown PO, Lieb JD (2003) Genomewide demarcation of RNA polymerase II transcription units revealed by physical fractionation of chromatin. Proc Natl Acad Sci USA 100:6364–6369
Okuwaki M, Verreault A (2004) Maintenance DNA methylation of nucleosome core particles. J Biol Chem 279:2904–2912
Ooi SK, Qiu C, Bernstein E, Li K, Jia D et al (2007) DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA. Nature 448:714–717
Rubin CM, VandeVoort CA, Teplitz RL, Schmid CW (1994) Alu repeated DNAs are differentially methylated in primate germ cells. Nucleic Acids Res 22:5121–5127
Sabo PJ, Kuehn MS, Thurman R, Johnson BE, Johnson EM et al (2006) Genome-scale mapping of DNase I sensitivity in vivo using tiling DNA microarrays. Nat Methods 3:511–518
Sakamoto H, Suzuki M, Abe T, Hosoyama T, Himeno E et al (2007) Cell type-specific methylation profiles occurring disproportionately in CpG-less regions that delineate developmental similarity. Genes Cells 12:1123–1132
Saxonov S, Berg P, Brutlag DL (2006) A genome-wide analysis of CpG dinucleotides in the human genome distinguishes two distinct classes of promoters. Proc Natl Acad Sci USA 103:1412–1417
Schones DE, Cui K, Cuddapah S, Roh TY, Barski A et al (2008) Dynamic regulation of nucleosome positioning in the human genome. Cell 132:887–898
Suzuki MM, Kerr AR, De Sousa D, Bird A (2007) CpG methylation is targeted to transcription units in an invertebrate genome. Genome Res 17:625–631
Takai D, Jones PA (2002) Comprehensive analysis of CpG islands in human chromosomes 21 and 22. Proc Natl Acad Sci USA 99:3740–3745
Vassetzky NS, Ten OA, Kramerov DA (2003) B1 and related SINEs in mammalian genomes. Gene 319:149–160
Weber M, Hellmann I, Stadler MB, Ramos L, Paabo S et al (2007) Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome. Nat Genet 39:457–466
Webster KE, O’Bryan MK, Fletcher S, Crewther PE, Aapola U et al (2005) Meiotic and epigenetic defects in Dnmt3L-knockout mouse spermatogenesis. Proc Natl Acad Sci USA 102:4068–4073
Yoder JA, Walsh CP, Bestor TH (1997) Cytosine methylation and the ecology of intragenomic parasites. Trends Genet 13:335–340
Acknowledgments
This work is supported by Einstein’s Center for Epigenomics and grants from the National Institutes of Health (NIH) to JMG (R01 HD044078 and R01 HG004401) and from the MRC and Wellcome Trust to AFS. JLG is supported by NIH MSTP Training Grant GM007288.
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Glass, J.L., Fazzari, M.J., Ferguson-Smith, A.C. et al. CG dinucleotide periodicities recognized by the Dnmt3a–Dnmt3L complex are distinctive at retroelements and imprinted domains. Mamm Genome 20, 633–643 (2009). https://doi.org/10.1007/s00335-009-9232-3
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DOI: https://doi.org/10.1007/s00335-009-9232-3