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Development of a bifunctional xylanase-cellulase chimera with enhanced activity on rice and barley straws using a modular xylanase and an endoglucanase procured from camel rumen metagenome

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Abstract

The camel rumen metagenome is an untapped source of glycoside hydrolases. In this study, novel genes encoding for a modular xylanase (XylC) and a cellulase (CelC) were isolated from a camel rumen metagenome and expressed in Escherichia coli BL21 (DE3). XylC with xylanase (Xyn), CBM, and carbohydrate esterase (CE) domains was characterized as a β-1,4-endoxylanase with remarkable catalytic activity on oat-spelt xylan (K cat = 2919 ± 57 s−1). The implication of XylC’s modular structure in its high catalytic activity was analyzed by truncation and fusion construction with CelC. The resulting fusions including Cel-CBM, Cel-CBM-CE, and Xyn-CBM-Cel showed remarkable enhancement in CMCase activity with K cat values of 742 ± 12, 1289 ± 34.5, and 2799 ± 51 s−1 compared to CelC with a K cat of 422 ± 3.5 s−1. It was also shown that the bifunctional Xyn-CBM-Cel with synergistic xylanase/cellulase activities was more efficient than XylC and CelC in hydrolysis of rice and barley straws.

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Acknowledgements

This work was supported by National Institute of Genetic Engineering and Biotechnology (NIGEB) (Grant Nos. 518 and 578).

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Authors and Affiliations

  1. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

    Kamran Khalili Ghadikolaei, Kambiz Akbari Noghabi & Hossein Shahbani Zahiri

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  1. Kamran Khalili Ghadikolaei
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  2. Kambiz Akbari Noghabi
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  3. Hossein Shahbani Zahiri
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Correspondence to Hossein Shahbani Zahiri.

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Khalili Ghadikolaei, K., Akbari Noghabi, K. & Shahbani Zahiri, H. Development of a bifunctional xylanase-cellulase chimera with enhanced activity on rice and barley straws using a modular xylanase and an endoglucanase procured from camel rumen metagenome. Appl Microbiol Biotechnol 101, 6929–6939 (2017). https://doi.org/10.1007/s00253-017-8430-2

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  • DOI: https://doi.org/10.1007/s00253-017-8430-2

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