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Unsaturation of Mitochondrial Membrane Lipids is Related to Palmitate Oxidation in Subsarcolemmal and Intermyofibrillar Mitochondria

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Abstract

Membrane lipid composition is thought to influence the function of integral membrane proteins; however, the potential for lipid composition to influence overall mitochondrial long-chain fatty acids (LCFA) oxidation is currently unknown. Therefore, the naturally occurring variability of LCFA oxidation rates within subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria in muscles with varying oxidative potentials (heart → red → white) was utilized to examine this relationship. To this end, SS and IMF mitochondria were isolated and palmitate oxidation rates were compared to membrane phospholipid composition. Among tissues, rates of palmitate oxidation in mitochondria displayed a 2.5-fold range, creating the required range to determine potential relationships with membrane lipid composition. In general, the percent mole fraction of phospholipid head groups and major fatty acid subclasses were similar in all mitochondria studied. However, rates of palmitate oxidation were positively correlated with both the unsaturation index and relative abundance of cardiolipin within mitochondria (r = 0.57 and 0.49, respectively; p < 0.05). Thus, these results suggest that mitochondrial LCFA oxidation may be significantly influenced by the total unsaturation and percent mole fraction of cardiolipin of the mitochondrial membrane, whereas other indices of membrane structure (e.g., percent mole fraction of other predominant membrane phospholipids, chain length, and ratio of phosphatidylcholine to phosphatidylethanolamine) were not significantly correlated.

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Acknowledgments

This study was supported by the Natural Sciences and Engineering Research Council of Canada (G. P. H., P. J. L.). V. A. F. was supported by a Natural Sciences and Engineering Research Council of Canada Undergraduate Student Research Award.

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Authors and Affiliations

  1. Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada

    Graham P. Holloway

  2. Centre for Bone and Muscle Health, Faculty of Applied Health Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, ON, L2S 3A1, Canada

    Val Andrew Fajardo, Lauren McMeekin & Paul J. LeBlanc

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  1. Graham P. Holloway
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  2. Val Andrew Fajardo
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  4. Paul J. LeBlanc
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Correspondence to Paul J. LeBlanc.

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Holloway, G.P., Fajardo, V.A., McMeekin, L. et al. Unsaturation of Mitochondrial Membrane Lipids is Related to Palmitate Oxidation in Subsarcolemmal and Intermyofibrillar Mitochondria. J Membrane Biol 245, 165–176 (2012). https://doi.org/10.1007/s00232-012-9426-6

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  • DOI: https://doi.org/10.1007/s00232-012-9426-6

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