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Sphingobium tyrosinilyticum sp. nov., a tyrosine hydrolyzing bacterium isolated from Korean radish garden

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Abstract

A yellow-pigmented novel bacterial strain, MAH-12T, was isolated from a soil sample of Korean radish garden and was characterized using a polyphasic approach. Cells were Gram-staining negative, non-motile and rod-shaped. The strain was aerobic, catalase positive, optimum growth temperature and pH were 28–30 °C and 6.0, respectively. The novel strain is able to hydrolyze l-tyrosine, starch, esculin and 4-nitrophenyl-β-d-galactopyranoside. On the basis of 16S rRNA gene sequence analysis, strain MAH-12T belongs to the genus Sphingobium and is most closely related to several Sphingobium type strains (97.2–97.8%). In DNA–DNA hybridization tests, the DNA relatedness between strain MAH-12T and its closest phylogenetic neighbors was below 45.0%. The DNA G + C content was 64.0 mol% and the predominant respiratory quinone was ubiquinone-10. The major cellular fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C16:0. The DNA–DNA hybridization results and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain MAH-12T represented a novel species within the genus Sphingobium, for which the name Sphingobium tyrosinilyticum is proposed. The type strain is MAH-12T (= KACC 19297T = CGMCC 1.16225T). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain MAH-12T is KY964278 and the digital protologue database taxon number of strain MAH-12T is TA00463.

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Acknowledgements

This study was performed with the support of the Cooperative Research Program of the National Research Foundation of Korea Grant (Project No. NRF-2016R1A2B4014591) funded by the Korea government (MISP), Republic of Korea.

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Author notes

  1. Md. Amdadul Huq, Shahina Akter equally contributed to this work.

Authors and Affiliations

  1. Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea

    Md. Amdadul Huq, Jae-Hyun Yoon & Sun-Young Lee

  2. Department of Horticultural Life Science, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Shahina Akter

  3. Department of Biotechnology, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi

  4. Department of Food Science and Technology, Sejong University, Gwangjin-gu, Seoul, 143-747, Republic of Korea

    Sri Renukadevi Balusamy

  5. Department of Horticulture, Sunchon National University, Suncheon, 57922, Republic of Korea

    Sathishkumar Natarajan

Authors
  1. Md. Amdadul Huq
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  2. Shahina Akter
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  3. Muhammad Zubair Siddiqi
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  4. Sri Renukadevi Balusamy
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  5. Sathishkumar Natarajan
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  6. Jae-Hyun Yoon
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  7. Sun-Young Lee
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Corresponding authors

Correspondence to Md. Amdadul Huq or Sun-Young Lee.

Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank accession number for the 16S rRNA gene sequence of strains MAH-12T is KY964278.

Electronic supplementary material

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203_2018_1531_MOESM1_ESM.pptx

Supplementary Table 1. Negative properties of strain MAH-12T carried out by commercial test kits (API 20NE and API ZYM) (PPTX 50 KB)

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Supplementary material 3 (PDF 51 KB)

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Huq, M.A., Akter, S., Siddiqi, M.Z. et al. Sphingobium tyrosinilyticum sp. nov., a tyrosine hydrolyzing bacterium isolated from Korean radish garden. Arch Microbiol 200, 1143–1149 (2018). https://doi.org/10.1007/s00203-018-1531-1

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  • DOI: https://doi.org/10.1007/s00203-018-1531-1

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