The combination treatment system of cryosurgery and hyperthermia treatment was fabricated. The system consists of Peltier device, a power supply, a coolant chamber, Stirling cooler and a peristaltic pump. In the system fabrication, an aluminum box was mechanically worked and was attached to Stirling cooler and two pipes with a thermally-conductive bond. In the fabrication of the surgical probe, two pipes and Peltier device were respectively attached to two circular plates. The surgical probe was produced by fixing these plates to PVC pipe. Finally, the coolant chamber and the probe were connected utilizing the cold-resistant tube used at a peristaltic pump. Thus, the combination treatment system was fabricated. The system performance was evaluated using ethanol as a coolant in no-load running condition. During the test, a power was supplied to Peltier device and temperatures were measured. In the results of temperature measurement, the probe temperature reached to -45 degrees C and repeated the process of the cooling and the heating, alternately and continuously. This work was partly supported by JSPS Grants-in-Aid for Young Scientists (B) number 26820065.