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Simultaneous voltammetric detection of 5-hydroxyindole-3-acetic acid and 5-hydroxytryptamine using a glassy carbon electrode modified with conducting polymer and platinised carbon nanofibers

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Abstract

The authors describe a method for simultaneous voltammetric determination of 5-hydroxytryptamine (serotonin; 5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA). A glassy carbon electrode was modified with poly(pyrrole-3-carboxylic acid) and with platinised carbon nanofibers to obtain a sensor that can quantify 5-HT and 5-HIAA with detection limits of 10 nM and 20 nM, respectively. The peak currents, best measured at voltages of 170 mV and 500 mV (vs. Ag/AgCl) for 5-HT and 5-HIAA, increase linearly in the 0.01–100 μM concentration range for both analytes. The method was successfully applied to the quantitation of 5-HT and 5-HIAA in spiked artificial urine samples, and the sensor can be used up to 10 days.

A new electroanalytical device was developed for separation and quantitation of 5-hydroxytryptamine (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA), based on stripping square wave voltammetry, exploiting conducting polymer surfaces on platinised carbon nanofiber supports.

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Acknowledgements

This work was partially funded by the “SMARTCANCERSENS” project Marie Curie IRSES staff exchange programme - Grant Agreement PIRSES-GA 2012-318053.

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Authors and Affiliations

  1. Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, University of Sousse, 4003 IbnKhaldoun, Sousse, Tunisia

    Zina Fredj & MounirBen Ali

  2. NANOMISENE Lab, LR16CRMN01, Centre for Research on Microelectronics and Nanotechnology of Sousse, Technopole of Sousse, Sahloul, 4034, Sousse, Tunisia

    Zina Fredj & MounirBen Ali

  3. MiCRA Biodiagnostics, Centre of Applied Science for Health, Institute of Technology Tallaght, Tallaght, D24 FKT9, Dublin 24, Ireland

    Baljit Singh

  4. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

    Eithne Dempsey

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  1. Zina Fredj
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Correspondence to Eithne Dempsey.

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Fredj, Z., Ali, M., Singh, B. et al. Simultaneous voltammetric detection of 5-hydroxyindole-3-acetic acid and 5-hydroxytryptamine using a glassy carbon electrode modified with conducting polymer and platinised carbon nanofibers. Microchim Acta 185, 412 (2018). https://doi.org/10.1007/s00604-018-2949-5

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