Skip to main content
Log in

Vacuolar (lysosomal) trehalase ofSaccharomyces cerevisiae

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

In the yeastSaccharomyces cerevisiae thePEP4 gene product, protease A, is responsible for activating all soluble vacuolar (lysosomal) enzymes. These vacuolar enzymes remain inactive inpep4 mutants. Vacuolar trehalase activity was diminished in such mutants as well. This suggests that the vacuolar (lysosomal) trehalase is processed in a manner similar to other vacuolar enzymes inS. cerevisiae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature Cited

  1. Ammerer G, Hunter CP, Rothman JH, Saari GC, Valls LA, Stevens TH (1986)PEP4 gene ofSaccharomcyes cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol Cell Biol 6:2490–2499

    Google Scholar 

  2. Bradford M (1976) A rapid and sensitive method for quantitation of microgram quantities of proteins utilising the principle of protein-dye binding. Anal Biochem 72:248–254

    Google Scholar 

  3. Chan AH, Cotter DA (1980) An improved coupled assay for detecting glucose release by acid glucosidases. Microbios Lett 15:7–15

    Google Scholar 

  4. Jones E (1984) The synthesis and function of proteases inSaccharomyces: genetic approaches. Annu Rev Genet 18:233–270

    Google Scholar 

  5. Jones E, Zubenko G, Parker R, Hemmings B, Hasilik A (1981) Pleiotropic mutations ofSaccharomyces cerevisiae which cause deficiency for proteinases and other vacuolar enzymes. In: von Wettstein, Friis J, Kielland-Brandt M, Stenderup A (eds) Alfred Benzer symposium: molecular genetics in yeast. Copenhagen: Munksgaard, pp. 183–198

    Google Scholar 

  6. Londesborough J, Varimo K (1984) Characterization of two trehalases in baker's yeast. Biochem J 219:511–518

    Google Scholar 

  7. Matile P, Weimken A (1967) The vacuole as the lysosome of the yeast cell. Arch Microbiol 56:148–155

    Google Scholar 

  8. Thevelein JM (1984) Regulation of trehalose mobilization in fungi. Microbiol Rev 48:42–59

    Google Scholar 

  9. Wiemken A, Schellenberg M (1982) Does a cAMP-dependent phosphorylation initiate the transfer of trehalase from the cytosol into the vacuoles inSaccharomyces cerevisiae? FEBS Lett 150:329–331

    Google Scholar 

  10. Wiemken A, Schellenberg M, Urech K (1979) Vacuoles: the sole compartments of digestive enzymes in yeast (Saccharomyces cerevisiae)? Arch Microbiol 123:23–35

    Google Scholar 

  11. Woolford CA, Daniels LB, Park FJ, Jones EW, Van Arsdell JN, Innis MA (1986) ThePEP4 gene encodes an aspartyl protease implicated in the post-translational regulation ofSaccharomyces cerevisiae vacuolar hydrolases. Mol Cell Biol 6:2500–2510

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harris, S.D., Cotter, D.A. Vacuolar (lysosomal) trehalase ofSaccharomyces cerevisiae . Current Microbiology 15, 247–249 (1987). https://doi.org/10.1007/BF01589375

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01589375

Keywords

Navigation