Factors Affecting Fertilization and Embryonic Development During Intracytoplasmic Sperm Injection in Pigs

Michiko NAKAI; Naomi KASHIWAZAKI; Junya ITO; Naoki MAEDOMARI; Manabu OZAWA; Masao SHINO; Junko NOGUCHI; Hiroyuki KANEKO; Kazuhiro KIKUCHI

doi:10.1262/jrd.10-200E

Michiko NAKAI, Naomi KASHIWAZAKI, Junya ITO, Naoki MAEDOMARI, Manabu OZAWA, Masao SHINO, Junko NOGUCHI, Hiroyuki KANEKO, Kazuhiro KIKUCHI

Author information

Michiko NAKAI
Division of Animal Sciences, National Institute of Agrobiological Sciences
Naomi KASHIWAZAKI
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
Junya ITO
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
Naoki MAEDOMARI
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
Manabu OZAWA
Division of Animal Sciences, National Institute of Agrobiological Sciences
Masao SHINO
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
Junko NOGUCHI
Division of Animal Sciences, National Institute of Agrobiological Sciences
Hiroyuki KANEKO
Division of Animal Sciences, National Institute of Agrobiological Sciences
Kazuhiro KIKUCHI
Division of Animal Sciences, National Institute of Agrobiological Sciences

Keywords: Embryonic development, Fertilization, ICSI, Pig

JOURNAL FREE ACCESS

2011 Volume 57 Issue 2 Pages 183-187

DOI https://doi.org/10.1262/jrd.10-200E

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Abstract

In intracytoplasmic sperm injection (ICSI) technique, a sperm was injected into ooplasm directly using a glass pipette. The fertilization physiology in ICSI is considered quite different from that of the natural fertilization. The different mechanisms for fertilization may be the causes of various results in ICSI. In this paper, we focus on the state of sperm membranes, nuclear or DNA integrity during ICSI procedure and discuss the influence of these factors on fertilization and embryonic development. We also introduce some examples in application of ICSI for new technologies in pigs.

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