Issue 10, 2007
From the journal:

Lab on a Chip

Cargo pick-up from engineered loading stations by kinesin driven molecular shuttles†

Christian Brunner,a   Christian Wahnesa  and  Viola Vogel*a  

* Corresponding authors

a Laboratory for Biologically Oriented Materials, Department of Materials, ETH Zürich, Hönggerberg, Zürich, Switzerland

Abstract

Exploiting biological motors ex vivo to transport and distribute cargo with high spatial control, as done by cells, requires that we learn how molecular shuttles (microtubules propelled by kinesin) can pick up cargo from defined surface regions (loading stations). The main challenge of building microfabricated cargo loading stations is to adjust the sum of non-covalent interactions such that the station stably holds on to the cargo under static conditions, but allows for transfer when a gliding microtubule collides with station-bound cargo and starts to pull on it. Successful pick-up of cargo could be observed using biotin–anti-biotin interactions and hybridized oligonucleotides. The effect of different tethering chemistries on the efficiency of cargo pick-up was tested.

Graphical abstract: Cargo pick-up from engineered loading stations by kinesin driven molecular shuttles

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Article information

DOI
https://doi.org/10.1039/B707301A
Article type
Paper
Submitted
15 May 2007
Accepted
03 Jul 2007
First published
25 Jul 2007

Lab Chip, 2007,7, 1263-1271

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Cargo pick-up from engineered loading stations by kinesin driven molecular shuttles

C. Brunner, C. Wahnes and V. Vogel, Lab Chip, 2007, 7, 1263 DOI: 10.1039/B707301A

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