Abstract
CORTICOSTERONE, the principal adrenal steroid in the rat,, is retained by cell nuclei in the brain of adrenalectomized rats by a process specific to the corticosterone structure1. Nuclear binding of hormone is greatest in the hippocampus, where we have previously found the highest tissue concentration of radioactive hormone, but is also significant throughout the rest of the brain1,2. The binding of steroid hormone to cell nuclei in other target tissues, indicates that the hormone receptor is protein which can be extracted from the nuclei by moderate salt concentrations3–6. We now present evidence that hormone within brain cell nuclei is bound to a macromolecular component, probably protein, which can be extracted from isolated nuclei by 0.4 M NaCl.
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MCEWEN, B., PLAPINGER, L. Association of 3H Corticosterone-1,2 with Macromolecules extracted from Brain Cell Nuclei. Nature 226, 263–265 (1970). https://doi.org/10.1038/226263a0
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DOI: https://doi.org/10.1038/226263a0
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