Skip to main content
SpringerLink
Log in

Effect of inhibitors of ethylene biosynthesis and signal transduction pathway on the multiplication of in vitro-grown Hancornia speciosa

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Initiation and elongation of lateral buds is stimulated in in vitro-grown shoots of Hancornia speciosa(a tropical fruit tree) by high temperature (35 °C), which is associated with the plant's reduced ability to release ethylene. However, no increase in either lateral shoot elongation or multiplication rate of H. speciosa shoots growing in vitro at non-shoot inducing temperature (31 °C) was associated with the presence of inhibitors of ethylene biosynthesis, such as α-amino isobutyric acid and cobalt ions or (aminooxy) acetic acid. Likewise, no increase in the multiplication rate was associated with aminoethoxyvinylglycine (AVG), another inhibitor of ethylene biosynthesis. In addition, stimulation of lateral shoot elongation in H. speciosa shoots grown at non shoot-inducing temperature was achieved when two inhibitors of the ethylene signal transduction pathway, silver thiosulphate (5–10 μM Ag++) and 1-methylcyclopropene (90 nl l−1) were present in the culture medium or in the culture vessel-headspace, respectively. However, increased multiplication only occurred with the 1-methylcyclopropene-treated shoots. Thus, lateral bud development with 1-methylcyclopropene could be used to enhance H. speciosa multiplication in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Armstrong J, Lemos EEP, Zobayed SMA, Justin SHFW & Armstrong W (1997) A humidity-induced convective through flow ventilation system benefits Annona squamosa L. explants and coconut calloid. Ann. Bot. 79: 31-40

    Article  Google Scholar 

  • Biddington NL (1992) The influence of ethylene in plant tissue culture. Pl. Growth Reg. 11: 173-187

    Article  CAS  Google Scholar 

  • Bleecker AB & Schaller GE (1996) The mechanism of ethylene perception. Plant Physiol. 111: 653-660

    PubMed  CAS  Google Scholar 

  • Gaspar T, Kevers C, Bouillenne H, Maziere Y & Barbe J-P (1989) Ethylene production in relation to rose micropropagation through axillary budding. In: Clijsters H et al. (eds) Biochemical and Physiological Aspects of Ethylene Production in Lower and Higher Plants (pp 303-312). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • George EF & Sherrington PD (1984) Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Exegetics Ltd, Eversley

    Google Scholar 

  • Hall WC, Aho HM, Berry AW, Cowan DS, Harpham NVJ, Holland MG, Moskov I Ye, Novikova G & Smith AR (1993) Ethylene receptors. In: Pech JC et al., (eds) Cellular and Molecular Aspects of the Plant Hormone Ethylene (pp 168-173). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Kieber JJ (1997) The ethylene response pathway in arabidopsis. Annu. Rev. Plant Physiol. Mol. Biol. 48: 277-296

    Article  CAS  Google Scholar 

  • Karhu ST (1997a) Axillary shoot proliferation of blue honeysuckle. Plant Cell Tiss. Org. Cult. 48: 195-201

    Article  Google Scholar 

  • Karhu ST (1997b) Sugar use in relation to shoot induction by sorbitol and cytokinin in apple. J. Am. Soc. Hortic. Sci. 122: 476-480

    CAS  Google Scholar 

  • Keller CP & Van Volkenburgh E (1997) Auxin-induced epinasty of tobacco leaf tissues. Plant Physiol. 113: 603-610

    PubMed  CAS  Google Scholar 

  • Lambardi M, Benelli C & Fabbri A (1997) In vitro axillary shoot proliferation of apple rootstocks under different ethylene conditions. In Vitro Cell. Dev. Biol.-Plant. 33: 70-74

    CAS  Google Scholar 

  • Li-Chun J & Li CJ (1997) Role of ethylene in apical dominance of pea plants. Acta-Phytophysiol. Sinica 23: 283-287

    Google Scholar 

  • Marino G, Berardi G & Ancherani M (1995) The effect of the type of closure on the gas composition of the headspace and the growth of GF 677 peach × almond rootstock cell suspension cultures. In Vitro Cell. Dev. Biol.-Plant 31: 207-210

    Google Scholar 

  • Martin MN, Cohen JD & Saftner RA (1995) A new 1-aminocyclopropane-1-carboxylic acid-conjugating activity in tomato fruit. Plant Physiol. 109: 917-926

    Article  PubMed  CAS  Google Scholar 

  • Matthys D, Gielis J & Debergh P (1995) Ethylene. In: Aitken-Christie J et al. (eds) Automation and Environmental Control in Plant Tissue Culture (pp 473-491). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Mulkey TJ, Kuzmanoff KM & Evans ML (1982) Promotion of growth and hydrogen ion efflux by auxin in roots of maize pretreated with ethylene biosynthesis inhibitors. Plant Physiol. 70: 186-188

    PubMed  CAS  Google Scholar 

  • Murashige T & Skoog F (1962) A revised method for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497

    Article  CAS  Google Scholar 

  • Pereira-Netto AB & McCown BH (1999) Thermally induced changes in shoot morphology of Hancornia speciosa microcultures: evidence of mediation by ethylene. Tree Physiol. 19: 733-740

    PubMed  Google Scholar 

  • Saltveit-Jr ME & Larson RA (1983) Effect of mechanical stress and inhibitors of protein synthesis on leaf epinasty in mechanically stressed poinsettia plants. J. Amer. Soc. Hort. Sci. 108: 253-257

    Google Scholar 

  • Shekhawat NS, Rathore T S, Singh RP, Deora NS & Rao SR (1993) Factors affecting in vitro clonal propagation of Prosopis cineraria. Pl. Growth Reg. 12: 273-280

    Article  CAS  Google Scholar 

  • Sisler EC (1991) Ethylene-binding components in plants. In:Mattoo AK & Suttle JC (eds.) The Plant Hormone Ethylene (pp. 81-99). CRC Press, Boca Raton

    Google Scholar 

  • Sisler EC, Dupille E & Serek M (1996a)Effect of 1-methylcyclopropene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations. Plant Growth Regul. 18: 79-86

    Article  CAS  Google Scholar 

  • Sisler EC, Serek M & Dupille E (1996b) Comparison of cyclopropene, 1-methylcyclopropene and 3,3-dimethylcyclopropene as ethylene antagonists in plants. Plant Growth Regul. 18: 169-174

    Article  CAS  Google Scholar 

  • Sisler EC & Serek M (1997) Inhibitors of ethylene responses in plants at the receptor level: Recent developments. Physiol. Plant. 100: 577-582

    Article  CAS  Google Scholar 

  • Veen H & Overbeek JHM (1989) The action of silver thiosulphate in carnation petals. In: Clijsters H et al. (eds) Biochemical and Physiological Aspects of Ethylene Production in Lower and Higher Plants (pp 109-117). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Vioque B & Castellano JM(1994) Extraction and biochemical characterization of 1-aminocyclopropane-1-carboxylic acid oxidase from pear. Physiol. Plant. 90: 334-338

    Article  CAS  Google Scholar 

  • Woltering EJ, Somhorst D & Van Der Veer P (1995) The role of ethylene in interorgan signaling during flower senescence. Plant Physiol. 109: 1219-1225

    PubMed  CAS  Google Scholar 

  • Yang SF & Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Ann. Rev. Plant Physiol. 35: 155-189

    CAS  Google Scholar 

  • Yu Y-B, Adams DO & Yang SF (1980) Inhibition of ethylene production by 2,4-dinitrophenol and high temperature. Plant Physiol. 66: 286-290

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany-SCB, Centro Politécnico, University of Parana, C.P. 19031, 81531-970, Curitiba, PR, Brazil

    Adaucto B. Pereira-Netto

Authors
  1. Adaucto B. Pereira-Netto
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pereira-Netto, A.B. Effect of inhibitors of ethylene biosynthesis and signal transduction pathway on the multiplication of in vitro-grown Hancornia speciosa. Plant Cell, Tissue and Organ Culture 66, 1–7 (2001). https://doi.org/10.1023/A:1010699922346

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010699922346

Search

Navigation