Skip to main content
Log in

Menadione inhibits MIBG uptake in two neuroendocrine cell lines

  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

In this paper we report on our studies of the effect of menadione on the uptake of MIBG in the neuroendocrine cell lines PC12 and SK-N-SH. Menadione inhibits the uptake of MIBG in both cell lines in a dose-dependent manner. Inhibition of MIBG uptake is most pronounced in the PC12 cell line. Comparison of the inhibitory action of menadione on the uptake and retention of MIBG with that of imipramine and reserpine suggests that menadione inhibits uptake 1 mediated uptake as well as granular storage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wieland DM, Wu JI, Brown LW, Magner TJ, Swanson DP, Beierwaltes WH: Radiolabeled adrenergic neuroblocking agents: adrenomedullary imaging with [131I] iodobenzylguanidine. J Nucl Med 21: 349–353, 1980

    Google Scholar 

  2. Hoefnagel CA, Voûte PA, de Kraker J, Marcuse HR: Total-body scintigraphy with 13I-meta-iodobenzylguanidine for detection of neuroblastoma. Diagn Imag Clin Med 54: 21–27, 1985

    Google Scholar 

  3. Hoefnagel CA, Voûte PA, de Kraker J, Marcuse HR: Radionuclide diagnosis and therapy of neural crest tumors using iodine-131 meta iodobenzylguanidine. J Nucl Med 28: 308–314, 1987

    Google Scholar 

  4. Voûte PA, van der Kleij AJ, De Kraker J, Hoefnagel CA, Tiel-van Buul MMC, van Gennip H: Clinical experience with radiation enhancement by hyperbaric oxygen in children with recurrent neuroblastoma stage IV. Eur J Cancer 31A: 596–600, 1995

    Google Scholar 

  5. Vaidyanathan G, Strickland Z: Meta-[211At] Astatobenzylguanidine: further evaluation of a potential therapeutic agent. Int J Cancer 57: 908–913, 1994

    Google Scholar 

  6. Armour A, Mairs RJ, Gaze MN, Wheldon TE: Modification of MIBG uptake in neuroblastoma cells by elevated temperature. Br J Cancer 70: 445–448, 1994

    Google Scholar 

  7. Bosin TR: Oxidant stress stimulates active transport of serotonin by platelets: J Pharmacol Exp Ther 248: 67–72, 1989

    Google Scholar 

  8. Bosin TR, Schaltenbrand L: Stimulation of platelet serotonin transport by substituted 1, 4 Naphtoquinone-induced oxidant stress. Biochem Pharmacol 41: 967–974, 1991

    Google Scholar 

  9. Schickler M, Knobler H, Avraham KB, Elroy-Stein O, Groner Y: Diminished serotonin uptake in platelets of transgenic mice with increased Cu/Zn-Superoxidedismutase activity. EMBO J 8: 1385–1392, 1989

    Google Scholar 

  10. Elroy-Stein O, Groner Y: Impaired neurontransmitter uptake in PC12 cells overexpressing human Cu/Zn-Superoxidedismutase–implication for gene dosage effects in Down Syndrome. Cell 52: 259–267, 1988

    Google Scholar 

  11. Thor H, Smith MT, Hartzell P, Bellomo G, Jewell S, Orrenius S: The metabolism of menadione (2-methyl 1, 4-naphtoquinone) by isolated hypatocytes. J Biol Chem 257: 124–12425, 1982

    Google Scholar 

  12. Mertens JJR, Vanryckeghem W, Carlsen L: Cu(I) supported isotopic exchange of acetylbound iodide, new future for fast high yield labeling. In: Cox P (ed) Progress in Radiopharmacy, Proceedings of the Second European Symposium on Radiopharmacy and Radiopharmaceuticals. Martinus Nijhoff, The Hague, 1986, p 101

    Google Scholar 

  13. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD: Measurement of protein using bicinchoninc acid. Ann Biochem 150: 76–85, 1985

    Google Scholar 

  14. Smets LA, Janssen M, Rutgers M, Ritzen K, Buitenhuis C: Pharmacokinetics and intracellular distrubution of the tumor-targeted radiopharmaceutical MIBG in SK-N-SH neuroblastoma and PC12 pheochromocytoma cells. Int J Cancer 48: 609–615, 1991

    Google Scholar 

  15. Smets LA, Janssen M, Metwally E, Loesberg C: Extragranular storage of the neuron blocking agent MIBG in human neuroblastoma cells. Biochem Pharmacol 39: 1959–1964, 1990

    Google Scholar 

  16. Walsh SP, Patterson JW: Effects of hydrogen peroxide oxidation and calcium channel blockers on the equatorial current of the frog lens. Exp Eye Res 58: 257–266, 1994

    Google Scholar 

  17. Glowniak JV, Kilty JE, Amara SG, Hoffman BJ Turner FE: Evaluation of MIBG uptake by the norepinephrine, dopamine and serotonin transporters. J Nucl Med 34: 1140–1146, 1993

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cornelissen, J., Tytgat, G., van den Brug, M. et al. Menadione inhibits MIBG uptake in two neuroendocrine cell lines. J Neurooncol 31, 147–151 (1997). https://doi.org/10.1023/A:1005718421774

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005718421774

Navigation