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Effect of Different Flavonoids on Collagen Synthesis in Human Fibroblasts

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Abstract

In this study, we discovered that flavonoids belonging to the subclasses: (flavanone, flavone, and flavonol) display differential effects on the synthesis of collagen in human dermal fibroblasts. At 80 μg/ml flavonoids quercetin-3,3′,4′, 5,7-pentahydroxyflavone, 3-methyl quercetin, and 7-hydroxyflavone significantly decreased the total protein concentration which was a direct consequence of their cytotoxic effect, while naringenin exhibited no effect on total collagen and total protein concentration. Quercetin-3,3′4′,7-tetramethyl ether, 4′-hydroxyflavanone, flavanone, and fisetin significantly decreased collagen concentration while morin, rutin, and chrysin increased collagen concentration without changing the overall protein concentration. The initial screening performed in this study enables the identification of compounds that exert significant effects on fibroblast function and show potential as starting material for pharmaceutical preparations targeted against various disorders centered around disturbed collagen metabolism.

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Acknowledgments

This study was performed within the Masters degree program at the Faculty of Medicine and Pharmaceutical Sciences, University of Antwerp, Belgium. The authors would like to thank Prof. Karen Reiser, Department of Internal Medicine, University of California at Davis, for helping in analysing collagen data.

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Authors and Affiliations

  1. Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia

    TAMARA STIPCEVIC

  2. Department of Molecular Genetics, Rudjer Boskovic Institute, Zagreb, Croatia

    JASENKA PILJAC

  3. Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium

    DIRK VANDEN BERGHE

Authors
  1. TAMARA STIPCEVIC
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  2. JASENKA PILJAC
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  3. DIRK VANDEN BERGHE
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Correspondence to TAMARA STIPCEVIC.

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STIPCEVIC, T., PILJAC, J. & BERGHE, D.V. Effect of Different Flavonoids on Collagen Synthesis in Human Fibroblasts. Plant Foods Hum Nutr 61, 27–32 (2006). https://doi.org/10.1007/s11130-006-0006-8

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  • DOI: https://doi.org/10.1007/s11130-006-0006-8

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