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Direct observation of basic protein HEWL as an oxoanions receptor by electrospray ionization mass spectrometry

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Abstract

Positive ion mode of electrospray ionization mass spectrometry (ESI-MS) has been used for the detection and study of protein interaction. From the measurement of molecular mass of the intact complex and individual binding partners, the binding stoichiometry can be derived. In our work, one basic protein, hen egg white lysozyme (HEWL) as an anion receptor shows high sensitivity and selectivity responses to most oxoanions detected by ESI-MS. But neutral protein, such as insulin, does not response to anions. It was found that HSO4 -, IO4 -, ClO4 -, H2PO4 -, HCO3 - and AcO- have strong affinity to interact with HEWL under present condition, butHSO3 -, NO3 -, Cl- and F- could not be trapped by HEWL. ESI-MS condition and concentration of anions areconsidered. This is an important evidence obtained by mass spectrometryfor the distribution of anion recognition with a native protein.

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Authors and Affiliations

  1. The Key Laboratory for Chemical Biology, Fujian Province, College of Chemistry and Engineering, Xiamen University, Xiamen, P.R. China

    Lifeng Yang, Lin Yu, Yan Liu, Yong Chen, Liming Hu & Yufen Zhao

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  1. Lifeng Yang
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  2. Lin Yu
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  3. Yan Liu
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  4. Yong Chen
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  5. Liming Hu
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  6. Yufen Zhao
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Correspondence to Yufen Zhao.

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Yang, L., Yu, L., Liu, Y. et al. Direct observation of basic protein HEWL as an oxoanions receptor by electrospray ionization mass spectrometry. Letters in Peptide Science 10, 89–92 (2003). https://doi.org/10.1023/B:LIPS.0000032368.82164.a5

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  • DOI: https://doi.org/10.1023/B:LIPS.0000032368.82164.a5

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