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Productivity analysis of outdoor chemostat culture in tubular air-lift photobioreactors

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Abstract

Net productivity and biomass night losses in outdoor chemostat cultures ofPhaeodactylum tricornutum were analyzed in two tubular airlift photobioreactors at different dilution rates, photobioreactor surface/volume ratios and incident solar irradiance. In addition, an approximate model for the estimation of light profile and average irradiance inside outdoor tubular photobioreactors was proposed. In both photobioreactors, biomass productivity increased with dilution rate and daily incident solar radiation except at the highest incident solar irradiances and dilution rates, when photoinhibition effect was observed in the middle of the day. Variation of estimated average irradiance vs mean incident irradiance showed two effects: first, the outdoor cultures are adapted to average irradiance, and second, simultaneous photolimitation and photoinhibition took place at all assayed culture conditions, the extent of this phenomena being a function of the (incident)1 irradiance and light regime inside the culture. Productivity ranged between 0.50 and 2.04 g L−1 d−1 in the tubular photobioreactor with the lower surface/volume ratio (S/V = 77.5 m−1) and between 1.08 and 2.76 g L−1 d−1 in the other (S/V = 122.0 m−1). The optimum dilution rate was 0.040 h−1 in both reactors. Night-time biomass losses were a function of the average irradiance inside the culture, being lower in TPB0.03 than TPB0.06, due to a better light regime in the first. In both photobioreactors, biomass night losses strongly decreased when the photoinhibition effect was pronounced. However, net biomass productivity also decreased due to lower biomass generation during the day. Thus, optimum culture conditions were obtained when photolimitation and photoinhibition were balanced.

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References

  • Bajpai P, Bajpai P (1993) Eicosapentaenoic acid (EPA) production from microrganisms: a review. J. Biotechnol. 30: 161–183.

    PubMed  Google Scholar 

  • Cohen Z, Vonshak A, Richmond A (1988) Effect of environmental conditions on fatty acid composition of the red algaPorphyridium cruentum: Correlation to growth rate. J. Phycol. 24: 328–332.

    Google Scholar 

  • Duffie JA, Beckman WA (1980) Solar Engineering of Thermal Processes. John Wiley & Sons, New York.

    Google Scholar 

  • Gudin C, Chaumont D (1983) Solar biotechnology study and development of tubular solar receptors for controlled production of photosynthetic cellular biomass. In Palz W, Pirrwitz D (eds), Proceedings of the Workshop and E. C. Contractor's Meeting in Capri. D. Reidel Publishing Company, Dordrecht 184–193.

    Google Scholar 

  • Goldman JC (1979) Outdoor algal mass culture. II. Photosynthetic field limitations. Wat. Res. 13: 119–160.

    Google Scholar 

  • Grobbelaar JU, Soeder CJ (1985) Respiration losses in planktonic green algae cultivated in raceway ponds. J. Plankton Res. 7: 497–506.

    Google Scholar 

  • Grobbelaar JU, Kroon BMA, Burger-Wiersma T, Mur LR (1992) Influence of medium frequency light/dark cycles of equal duration on the photosynthesis and respiration ofChlorella pyrenoidosa. Hydrobiologia 238: 53–62.

    Google Scholar 

  • Gudin C, Chaumont D (1983). Solar biotechnology study and development of tubular solar receptors for controlled production of photosynthetic cellular biomass. In Palz W, Pirrwitz D (eds), Proceedings of the Workshop and E. C. Contractors' Meeting in Capri. Reidel Publ. Co., Dordrecht, 184–193.

    Google Scholar 

  • Gudin C, Therpenier C (1986) Bioconversion of solar energy into organic chemicals by microalgae. Advances in Biotechnological Processes 6: 73–110.

    Google Scholar 

  • Hansmann E (1973). Pigment analysis. En Hanbook of phycological methods, culture methods and growth measurements. Stein J. R. (ed.), Cambridge University Press, London, 359–368.

    Google Scholar 

  • Hu Q, Guterman H, Richmond A (1996) A flat inclined modular photobioreactor (FIMP) for outdoor mass cultivation of photoautotrophs. Biotechnol. Bioengng (in press).

  • Incropera FP, Thomas JF (1978) A model for solar radiation conversion to algae in shallow pond. Solar Energy 20: 157–165.

    Google Scholar 

  • Jensen S, Knutsen G (1993) Influence of light and temperature on photoinhibition of photosynthesis inSpirulina platensis. J. appl. Phycol. 5: 495–504.

    Google Scholar 

  • Kaixian Q, Borowitzka MA (1992). Light and deficiency effects on the growth and composition ofPhaeodacrylum tricornutum. Appl. Biochem. Biotechnol. 38: 93–103.

    Google Scholar 

  • Lee YK, Low CS (1991) Effect of photobioreactor inclination on the biomass productivity of an algal culture. Biotech. Bioengng 38: 995–1000.

    Google Scholar 

  • Lee YK, Low CS (1992) Productivity of outdoor algal cultures in enclosed tubular photobioreactor. Biotech. Bioengng 40: 1119–1122.

    Google Scholar 

  • Liu BYH, Jordan RC (1960). The interrelationship and characteristic distribution of direct, diffuse and total solar radiation. Solar Energy 7: 53–74.

    Google Scholar 

  • Mann JE, Myers J (1968). On pigments, growth and photosynthesis ofPhaeodactylum tricornutum. J. Phycol. 4: 349–355.

    Google Scholar 

  • Molina Grima E, García Camacho F, Sánchez Pérez JA, Acién Fernández FG, Fernández Sevilla JM, Valdés Sanz F (1994d) Effect of dilution rate on eicosapentaenoic acid productivity ofPhaeodactylum tricornutum UTEX 640 in outdoor chemostat culture. Biotechnology Letters 16: 1035–1040.

    Google Scholar 

  • Molina Grima E, García Camacho F, Sánchez Pérez JA, Fernández Sevilla JM, Acién Fernández FG, Contreras Gómez A (1994a) A mathematical model of microalgal growth in light limited chemostat culture. J. Chem. Tech. Biotechnol. 61: 167–173.

    Google Scholar 

  • Molina Grima E, Sánchez Pérez JA, García Camacho F, Fernández Sevilla JM, Acién Fernández FG, Urda Cardona J (1994b) Biomass and eicosapentaenoic acid productivities from an outdoor batch culture ofPhaeodactylum tricornutum UTEX 640 in an airlift photobioreactor. Appl. Microbiol. Biotechnol. 42: 658–663.

    Google Scholar 

  • Molina Grima E, Sánchez Pérez JA, García Camacho F, García Sánchez JL, Acién Fernández FG, López Alonso D (1994c) Outdoor culture ofIsochrysis galbana ALII-4 in a closed tubular photobioreactor. J. Biotechnol. 37: 159–166.

    Google Scholar 

  • Molina Grima E, Sánchez Pérez JA, García Camacho F, García Sánchez JL, Fernández Sevilla JM (1995) Variation of fatty acid profile with solar cycle in outdoor chemostat culture ofIsochrysis galbana ALII-4. J. appl. Phycol. 7: 129–134.

    Google Scholar 

  • Moo-Young M, Blanch HW (1981) Design of biochemical reactors: Mass transfer criteria for simple and complex systems. Adv. Biochem. Engngng 19: 1–69.

    Google Scholar 

  • Philipps JN, Myers J (1954) Measurement of algal growth under controlled steady-state conditions. Plant Physiol. 29: 152v161.

    Google Scholar 

  • Richmond A (1990) Large scale microalgal culture and applications. In Round FE, Chapman (eds), Progress in Phycological Research, Vol 7 Biopress Ltd, 269–329.

  • Richmond A, Boussiba S, Vonshak A, Kopel R (1993) A new tubular reactor for mass production of microalgae outdoors. J. appl. Phycol. 5: 327–332.

    Google Scholar 

  • Stitt M (1986) Limitation of photosynthesis by carbon metabolism. Plant Physiol. 81: 1115–1122.

    Google Scholar 

  • Terry KL (1986) Photosynthesis in modulated light: Quantitative dependence of photosynthetic enhancement on flashing rate. Biotechnol. Bioengng. 28: 988–995.

    Google Scholar 

  • Torzillo G, Sacchi A, Materassi R (1991) Temperature as an important factor affecting productivity and night biomass loss inSpirulina platensis grown outdoors in tubular photobioreactors. Bioresource Technol. 38: 95–100.

    Google Scholar 

  • Tredici MR, Carlozzi P, Chini Zitelli G, Materassi R (1991) A vertical alveolar panel (VAP) for outdoor mass cultivation of microalgae and cyanobacteria. Bioresource Technology 38: 153–159.

    Google Scholar 

  • Vonshak A, Guy R (1992) Photoadaptation, photoinhibition and productivity in the blue-green alga,Spirulina platensis, grown outdoors. Plant, Cell and Environment 15: 613–616.

    Google Scholar 

  • Whyte JN (1987) Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves. Aquaculture 60: 231–241.

    Google Scholar 

  • Wolfram S (1991) Mathematica. A system for doing mathematics by computer. In: Wolfram S (ed.). Addison-Wesley Publishing Co., Redwood City, CA.

  • Yaroslavtzev IN (1953) Distribution of the energetical and light intensity of diffuse atmospheric radiation over the celestial sphere. Bulletin of Leningrad University 5.

  • Yongmanitchai W, Ward O (1991) Growth of and Omega-3 fatty acid production byPhaeodactylum tricornutum under different culture conditions. Appl. envir. Microbiol. 57: 419–425.

    Google Scholar 

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Grima, E.M., Pérez, J.A.S., Camacho, F.G. et al. Productivity analysis of outdoor chemostat culture in tubular air-lift photobioreactors. J Appl Phycol 8, 369–380 (1996). https://doi.org/10.1007/BF02178580

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  • DOI: https://doi.org/10.1007/BF02178580

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