Abstract
We investigated to what extent south-exposed leaves (E-leaves) of the evergreen ivy (Hedera helix L.) growing in the shadow of two deciduous trees suffered from photoinhibition of photosynthesis when leaf-shedding started in autumn. Since air temperatures drop concomitantly with increase in light levels, changes in photosynthetic parameters (apparent quantum yield, Φ i and maximal photosynthetic capacity of O2 evolution, Pmax; chlorophyll-a fluorescence at room temperature) as well as pigment composition were compared with those in north-exposed leaves of the same clone (N-leaves; photosynthetic photon flux density PPFD< 100 μmol · m−2 · s−2) and phenotypic sun leaves (S-leaves; PPFD up to 2000 μmol · m−2 · s−1).
In leaves exposed to drastic light changes during winter (E-leaves) strong photoinhibition of photosynthesis could be observed as soon as the incident PPFD increased in autumn. In contrast, in N-leaves the ratio of variable fluorescence to maximum fluorescence (FV/FMm) and Φ i did not decline appreciably prior to severe frosts (up to -12° C) in January. At this time, Φ i was reduced to a similar extent in all leaves, from about 0.073 μmol O2 · μmol−1 photons before stress to about 0.020. Changes in Φ i were linearly correlated with changes in fv/fm (r = 0.955). The strong reduction in FV/FM on exposure to stress was caused by quenching in FM. The initial fluorescence (F0), however, was also quenched in all leaves. The diminished fluorescence yield was accompanied by an increase in zeaxanthin content. These effects indicate that winter stress in ivy primarily induces an increase in non-radiative energy-dissipation followed by “photoinhibitory damage” of PSII. Although a pronounced photooxidative bleaching of chloroplast pigments occurred in January (especially in E-leaves), photosynthetic parameters recovered completely in spring. Thus, the reduction in potential photosynthetic yield in winter may be up to three times greater in leaves subjected to increasing light levels than in leaves not exposed to a changing light environment.
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Abbreviations
- F0, FM :
-
initial and maximal fluorescence yield when all PSII centres are open and closed
- FV :
-
variable fluorescence (FM-F0)
- Pmax :
-
maximal photosynthetic capacity at 1000 umol · m−2 · s−1 PPFD and CO2 saturation
- PPFD:
-
photosynthetic photon flux density
- Φ i :
-
apparent quantum yield of photosynthetic O2 evolution
- E-leaves, N-leaves:
-
shade leaves exposed, not exposed to drastic light changes during winter
- S-leaves:
-
sun leaves from an open ivy stand
References
Albrecht, E. (1972) CO2-Gaswechsel und Hill-Aktivität von Hedera helix L. im Jahresverlauf unter Berücksichtigung klimatischer Faktoren. Photosynthetica 6, 240–246
Asada, K., Takahashi M. (1987) Production and scavenging of active oxygen in photosynthesis. In: Topics in photosynthesis, vol. 9: Photoinhibition, pp. 227–287, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam-New York-Oxford
Bauer, H., Kofler, R. (1987) Photosynthesis in frost-hardenend and frost-stressed leaves of Hedera helix L. Plant Cell Environ. 10, 339–346
Björkman, O. (1981) Responses to different quantum flux densities. In: Encyclopedia of plant physiology, N.S., vol. 12A: Physiological plant ecology I, pp. 57–107, Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H., eds. Springer, Berlin-Heidelberg-New York
Björkman, O., Demmig, B. (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origins. Planta 170, 489–504
Butler, W.L., Kitajima, M. (1975) Fluorescence quenching in photosystem II of chloroplasts. Biochim. Biophys. Acta 376, 116–125
Callahan, F.E., Becker, D.W., Cheniae, G.M. (1986) Studies on the photoactivation of the water-oxidizing enzyme. II. Characterization of weak light photoinhibition of PSII and its light-induced recovery. Plant Physiol. 82, 261–269
Cernusca, A. (1985) Der Einsatz von Taschencomputern für programmgesteuerte Datenerfassung, Auswertung und Versuchsüberwachung in der Ökologie. Verh. Ges. Ökol. 13, 369–377
Delieu, T., Walker, D.A. (1981) Polarographic measurement of photosynthetic oxygen evolution by leaf discs. New Phytol. 89, 165–178
Demmig-Adams, B. (1990) Carotenoids and photoprotection in plants: A role for the xanthophyll zeaxanthin. Biochim. Biophys. Acta 1020, 1–24
Demmig, B., Björkman, O. (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta 171, 171–184
Demmig, B., Winter, K., Krüger, A., Czygan, F.C. (1987) Photoin-hibition and zeaxanthin formation in intact leaves. A possible role of the xanthophyll cycle in the dissipation of excess light energy. Plant Physiol. 84, 218–224
Greer, D.H., Berry, J.A., Björkman, O. (1986) Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery. Planta 168, 253–260
Hager, A. (1957) Über den Einfluß klimatischer Faktoren auf den Blattfarbstoffgehalt höherer Pflanzen. Planta 49, 524–560
Hager, A. (1975) Die reversiblen, lichtabhängigen Xanthophyllumwandlungen im Chloroplasten. Ber. Dtsch. Bot. Ges. 88, 27–44
Hager, A., Meyer-Bertenrath, T. (1966) Die Isolierung und quantitative Bestimmung der Carotinoide und Chlorophylle von Blättern, Algen und isolierten Chloroplasten mit Hilfe dünnschichtchromatographischer Methoden. Planta 69, 198–217
Hager, A., Meyer-Bertenrath, T. (1967) Die Identifizierung der an Dünnschichten getrennten Carotinoide grüner Blätter und Algen. Planta 76, 149–168
Hoflacher, H., Bauer, H. (1982) Light acclimation in leaves of the juvenile and adult life phases of ivy (Hedera helix). Physiol. Plant. 56, 177–182
Kitajima, M., Butler, W.L. (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim. Biophys. Acta 376, 105–115
Krause, G.H. (1988) Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms. Physiol. Plant. 74, 566–574
Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. (1987) Topics in photosynthesis, vol. 9: Photoinhibition. Elsevier, Amsterdam-New York-Oxford
MacKinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322
Ögren, E. (1988) Photoinhibition of photosynthesis in willow leaves under field conditions. Planta 175, 229–236
Öquist, G., Ögren, E. (1985) Effects of winter stress on photosyn-thetic electron transport and energy distribution between the two photosystems of pine as assayed by chlorophyll fluorescence kinetics. Photosynth. Res. 7, 19–30
Pisek, A., Winkler, E. (1958) Assimilationsvermögen und Respiration der Fichte (Picea excelsa Link) in verschiedener Höhenlage und der Zirbe (Pinus cembra L.) an der alpinen Waldgrenze. Planta 51, 518–543
Powles, S.B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol. 35, 15–44
Samuelsson, G., Lönneborg, A., Gustafsson, P., Öquist, G. (1987) The susceptibility of photosynthesis to photoinhibition and the capacity of recovery in high and low light grown cyanobacteria, Anacystis nidulans. Plant Physiol. 83, 438–441
Schreiber, U. (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth. Res. 9, 261–272
Schreiber, U., Neubauer, C. (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: II. Partial control by the photosystem II donor side and possible ways of interpretation. Z. Naturforsch. 42c, 1255–1264
Schreiber, U., Schliwa, U., Bilger, W. (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10, 51–62
Senser, M., Beck, E. (1977) On the mechanisms of frost injury and frost hardening of spruce chloroplasts. Planta 137, 195–201
Siefermann, D., Yamamoto, H.Y. (1975) Properties of NADPH and oxygen-dependent zeaxanthin epoxidation in isolated chloroplasts. A transmembrane model for the violaxanthin cycle. Arch. Biochem. Biophys. 171, 70–77
Somersalo, S., Krause, G.H. (1990) Photoinhibition at chilling temperatures and effects of freezing stress on cold acclimated spinach leaves in the field. A fluorescence study. Physiol. Plant. 79, 617–622
Strand, M., Öquist, G. (1985) Inhibition of photosynthesis by freezing temperatures and high light levels in cold-acclimated seedlings of Scots pine (Pinus sylvestris). II. Effects on chlorophyll fluorescence at room temperature and 77K. Physiol. Plant. 65, 117–123
Strand, M., Öquist, G. (1888) Effects of frost hardening, dehardening and freezing stress on in vivo chlorophyll fluorescence of seedlings of Scots pine (Pinus sylvestris L.). Plant Cell Environ. 11, 231–238
Tranquillini, W. (1957) Standortsklima, Wasserbilanz und CO2Gaswechsel junger Zirben (Pinus cembra L.) an der alpinen Waldgrenze. Planta 49, 612–661
Yamamoto, H.Y. (1979) Biochemistry of the violaxanthin cycle in higher plants. Pure Appl. Chem. 51, 639–648
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Dedicated to Professor Otto Härtel on the occasion of his 80th birthday
This work was supported by the Austrian Fonds zur Förderung der wissenschaftlichen Forschung.
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Oberhuber, W., Bauer, H. Photoinhibition of photosynthesis under natural conditions in ivy (Hedera helix L.) growing in an understory of deciduous trees. Planta 185, 545–553 (1991). https://doi.org/10.1007/BF00202965
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DOI: https://doi.org/10.1007/BF00202965