Summary
Polyacrylamide gel electrophoresis (PAGE) of storage proteins (prolamines) was used to screen 64 landraces of wheat and barley from Nepal and the YemenArab Republic and two cultivars for comparison. Altogether 3168 single seeds were examined and the advantages gained by using the vertical slab gel method were recognised. The extent of variation present within populations of landraces could be assessed easily and rapidly using the methods described. Differences in ploidy levels of wheats were detected by PAGE and investigated. Suggestions are made for improvements in sampling strategies in hilly terrain.
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Damania, A.B., Porceddu, E. & Jackson, M.T. A rapid method for the evaluation of variation in germplasm collections of cereals using polyacrylamide gel electrophoresis. Euphytica 32, 877–883 (1983). https://doi.org/10.1007/BF00042169
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DOI: https://doi.org/10.1007/BF00042169