Regular ArticleTrypanosoma evansi: Cloning and Expression in Spodoptera fugiperda Insect Cells of the Diagnostic Antigen RoTat1.2
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Cited by (63)
Molecular prevalence, associated risk factors and genetic characterization of Trypanosoma evansi in camels
2023, Microbial PathogenesisA case of Trypanosoma evansi in a German Shepherd dog in Vietnam
2021, Parasitology InternationalEpidemiological investigations on Trypanosoma evansi infection in dromedary camels in the South of Algeria
2019, HeliyonCitation Excerpt :Given the low sensitivity of parasitological examinations in chronic infections, DNA amplification techniques, such as the polymerase chain reaction (PCR), are often applied as surrogate (Büscher, 2014). For surra, Trypanozoon-specific primers targeting satellite DNA or ribosomal DNA are the most sensitive (Gari et al., 2010; Masiga et al., 1992; Njiru et al., 2005) while the distinction between T. evansi type A and type B can be made with PCRs specific for the type A RoTat 1.2 gene and specific for type B minicircles (Claes et al., 2004; Njiru et al., 2006; Urakawa et al., 2001). In addition to parasitological or molecular diagnostics, serological tests are useful to provide indirect evidence of the presence of T. evansi in a susceptible population or individual.
Antigen detection ELISA: A sensitive and reliable tool for the detection of active infection of surra
2018, Acta TropicaCitation Excerpt :Several molecular diagnostics (Sengupta et al., 2010; Rudramurthy et al., 2013) and serological tests have been developed for the detection of trypanosomosis in animals which include, Suratex (Nantulya, 1994), CATT/T. evansi (Bajyana Songa and Hamers, 1988), LATEX/T. evansi, ELISA/ T. evansi (Verloo et al., 2000). Moreover, an attempt has been made by several research groups to improve serological (antibody detection) diagnostics using recombinant surface proteins such as variable surface glycoprotein (VSG) (Sengupta et al., 2012, 2014,2016; Urakawa et al., 2001), invariant surface glycoprotein (ISG) (Tran et al., 2008; Rudramurthy et al., 2015, 2017a,b) and flagellar protein (Ligi et al., 2016). The antibody detecting serological technique may not always detect true positive cases, because antibodies which are not found early in the infection may persit even after recovery or chemotherapy.
- 1
Present address: London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
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To whom correspondence should be addressed. Fax: 254-2-631499. E-mail: [email protected].