Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process

Contente, Martina Letizia; Farris, Stefano; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2019). Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process. Green chemistry, 21(12), pp. 3263-3266. Royal Society of Chemistry 10.1039/C9GC01374A

Preview

Text c9gc01374a.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (622kB) | Preview

To increase the uptake of biocatalytic processes by industry, it is essential to demonstrate the reliability of enzyme-based methodologies directly applied to the production of high value products. Here, a unique, efficient, and sustainable enzymatic platform for the multi-gram synthesis of melatonin, projected to generate around 1.5 billion U.S. dollars worldwide by 2021, and its analogues was developed. The system exploits the covalent immobilization of MsAcT (transferase from Mycobacterium smegmatis) onto agarose beads increasing the robustness and longevity of the immobilized biocatalyst. The fully-automated process deriving from the integration between biocatalysis and flow chemistry is designed to maximize the overall yields (58–92%) and reduce reaction times (5 min), overcoming the limitation often associated with bioprocesses and bridging the gap between lab scale and industrial production.

Interest & Impact

Downloads

166 since deposited on 09 Jul 2019
29 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item