Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY IN ANTIGENIC GLYCOPROTEIN FRACTIONS OBTAINED FROM THE CULTURE FILTRATE OF PSEUDOMONAS PSEUDOMALLEI
Eiko KONDO, Vimol PETKANCHANAPONG, Pimjai NAIGOWIT, Takeshi KURATA, Koomi KANAI
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1991 Volume 44 Issue 5-6 Pages 213-224

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Abstract

Pseudomonas pseudomallei, the causative microorganism of melioidosis, was grown in Mueller-Hinton liquid medium, and glycoprotein fractions were separated from the culture filtrate by ammonium sulfate precipitation, gel-filtration with Sephadex G-75, and column chromatography with DEAE-cellulose. The fractions revealed acid phosphatase activity, and reacted to the sera from melioidosis patient in gel-diffusion precipitation assay.

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