Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Analysis of Antioxidant Activity and Serotonin Derivatives in Safflower (Carthamus tinctorius L.) Germplasm Collected from Five Countries

국외 수집 홍화 유전자원의 항산화 활성 및 세로토닌 유도체 함량 분석

  • Jung, Yi Jin (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Assefa, Awraris Derbie (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Jae Eun (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Ho Sun (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Rhee, Ju Hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Sung, Jung Sook (Upland Crop Breeding, Research Division Department of Southern Area Crop Science, National Institute of Crop Science, RDA)
  • 정이진 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 아와리스 아세파 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이재은 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이호선 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이주희 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 성정숙 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과)
  • Received : 2019.05.23
  • Accepted : 2019.10.17
  • Published : 2019.10.31
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Abstract

In order to select potential plant resources as functional materials and natural antioxidants, we evaluated antioxidant activity and serotonin derivatives of safflower germplasm collected from five countries. N-(p-Coumaroyl) serotonin (CS) and N-feruloylserotonin (FS) were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total polyphenol content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric reducing antioxidant power (FRAP) and Reducing power (RP) assays. The TPC showed a range of 28.25 to $90.53{\mu}g$ gallic acid equivalent (GAE)/mg dried extract (DE). DPPH, ABTS, FRAP and RP assay were in the range of 18.76 to 93.98, 48.91 to 163.73, 3.80 to 132.29 and 26.32 to $80.08{\mu}g$ ascorbic acid equivalent (ASCE)/mg DE, respectively. Among the five countries, safflower seed collected from Iran had the highest levels of serotonin derivatives and antioxidant activities than other countries (p<0.05). CS showed high correlation with TPC, ABTS and DPPH (r=0.673,0.727,0.820), and FS showed high correlation with DPPH (r=0.740). Accessions IT321214 and IT321215 could be useful for development of new functional materials and could be used as a source of valuable natural antioxidant materials.

본 연구는 이란 등 5개국에서 수집한 홍화자원을 대상으로하여 천연항산화제로써 홍화종자의 항산화 활성을 평가하고, 세로토닌 유도체인N-(p-Coumaroyl) serotonin과N-feruloylserotonin의 함량을 측정하여 항산화 활성과의 상관관계를 조사하였다. 총폴리페놀함량은 $28.25{\sim}90.53{\mu}g\;GAE/mg\;DE$의 범위를 나타내었고, 색소화합물인 DPPH 라디칼과$ABTS^{+}$을 이용한 유리라디칼소거능을 측정하는 DPPH assay와 ABTS assay의 값은 18.76 ~ 93.98 및 $48.91{\sim}163.73{\mu}g\;ASCE/mg\;DE$의 범위를 나타내었다. 시료의 환원능을 측정하는 방법인 FRAP assay과 RP assay는 3.80 ~ 132.29 및 $26.32{\sim}80.08{\mu}g\;ASCE/mg\;DE$의 범위로 나타내었다. UHPLC를 이용하여 CS와 FS를 분석한 결과, CS 함량은 2.56 ~ 64.99 mg/g DE이고, FS 함량값은 1.92 ~ 65.36 mg/g DE이었다. 5개국 중 이란 원산종은 다른 나라에 비해 세로토닌 유도체 함량과 항산화 활성의 평균값이 높게 측정되었다. 항산화 활성과 세로토닌 유도체 함량간의 상관관계 분석 결과, CS는 TPC와 ABTS, DPPH와 높은 상관성을 보였고(r = 0.673, 0.727, 0.820), FS는 DPPH와 높은 상관성을 보였다(r = 0.740). RACI를 통하여 선발된 IT321214과 IT321215는 항산화 활성이 높은 자원으로 천연항산화제 소재 탐색 및 개발에 기초자료로 활용할 수 있을 것으로 생각된다.

Keywords

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