猪源狂犬病病毒N和G基因的序列分析

Analysis of N and G Gene of Rabies Virus Isolated from Pig

摘要: 通过对新分离的猪源狂犬病病毒GD-SH01株N基因和G基因进行序列分析，解析它们与其他狂犬病病毒N和G基因的差异.提取猪源狂犬病病毒GD-SH01株的总RNA，采用RT-PCR的方法扩增N基因和G基因，并与T载体连接，克隆至大肠埃希菌DH5α.测序后与国内外部分毒株的N基因和G基因进行核苷酸序列和氨基酸序列比对.与其他毒株相比，N基因核苷酸相似性为84.3%～98.0%；氨基酸相似性为92.5%～99.3%.G基因核苷酸相似性为80.4%～98.2%；氨基酸相似性为87.8%～99.6%.

Abstract: To understand the difference of the nucleoprotein (N) gene and glycoprotein (G) gene between the strain of pig origin and other rabies viruses. The N and G genes of rabies virus isolated from the brain of a rabid pig in Guangdong Province were amplified by RT-PCR, cloned, sequenced and compared with the genes of other rabies virus. The results showed that the sequence of nucleotides and amino acids of N genes of GD-SH01 shared 84.3%-98.0% and 92.5%-99.3% similarity，and G genes of GD-SH01 shared 80.4%-98.2% and 87.8%-99.6% similarity, respectively, compared to other viruses.