王声斌,黄自然,余让才,邹伟权,房师松. 宽皮橘蕉柑的胚性愈伤组织诱导、悬浮细胞系建立及植株再生[J]. 华南农业大学学报, 2002, 23(3): 52-55. DOI: 10.7671/j.issn.1001-411X.2002.03.015
    引用本文: 王声斌,黄自然,余让才,邹伟权,房师松. 宽皮橘蕉柑的胚性愈伤组织诱导、悬浮细胞系建立及植株再生[J]. 华南农业大学学报, 2002, 23(3): 52-55. DOI: 10.7671/j.issn.1001-411X.2002.03.015
    WANG Sheng bin 1,HUANG Zi ran 2,YU Rangcai 1,ZOU Weiquan 1,FANG Shi song 2. Studies on the Induction of Embryogenic Callus, Establishment of Cell Suspensions and Plant Regeneration in Citrus reticulata var. tankan Hayata[J]. Journal of South China Agricultural University, 2002, 23(3): 52-55. DOI: 10.7671/j.issn.1001-411X.2002.03.015
    Citation: WANG Sheng bin 1,HUANG Zi ran 2,YU Rangcai 1,ZOU Weiquan 1,FANG Shi song 2. Studies on the Induction of Embryogenic Callus, Establishment of Cell Suspensions and Plant Regeneration in Citrus reticulata var. tankan Hayata[J]. Journal of South China Agricultural University, 2002, 23(3): 52-55. DOI: 10.7671/j.issn.1001-411X.2002.03.015

    宽皮橘蕉柑的胚性愈伤组织诱导、悬浮细胞系建立及植株再生

    Studies on the Induction of Embryogenic Callus, Establishment of Cell Suspensions and Plant Regeneration in Citrus reticulata var. tankan Hayata

    • 摘要: 取蕉柑花后 5周幼果的幼胚接种于含不同浓度BA、KT与IAA组合的MT培养基上 ,结果表明 ,暗培养条件是蕉柑胚性愈伤组织诱导成功的关键 .胚状体在后期发育中畸形胚的比例很高 ,经切割后 ,在MT +BA2 .0mg·L-1+IAA0 5mg·L-1+蔗糖 0 0 3kg·L-1培养基上可诱导产生丛芽 ,诱导率为 2 6 7% .幼芽在 1/ 2MT +NAA 1 0mg·L-1培养基上生根率达到了 87 0 % .蕉柑胚性愈伤组织在MT液体培养基中不断地继代培养 ,可形成生长稳定的胚性悬浮细胞系 ,体胚诱导实验证明悬浮细胞系具有很强的体细胞胚胎发生能力 .

       

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