This study determined the relationship between health and physical fitness, and the contribution of each element in physical fitness to health for middle-aged and elderly women. A total of 1, 220 females aged 35 to 64 years participated in physical fitness tests and medical check ups. According to health status, the subjects were divided into two groups ; the healthy group (HG) and the unhealthy group (UG) . Multiple discriminant analysis was applied to the multivariate data. Correct discriminant probabilities of multiple discriminant functions to discriminate the HG and UG ranged from 63.9 to 83.3% among 6 age groups. These results suggested that there was a relatively strong relationship between health status and the level of physical fitness for middle-aged and elderly women. The change in correct discriminant probabilities indicated that degree of relationship would decrease stepwise with age. The contribution of each element of physical fitness to health status was also evaluated by Pearson's correlation coefficient. Age-related changes in the correlation coefficients between the discriminant score representing health status and performance on each physical fitness test demonstrated four different patterns. Therefore, special consideration should be given to the degree of contribution of each physical fitness element at each age level when developing exercise programs to promote health among middle-aged and elderly women.