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荣华, 李德强, 马敏伟, 豆腾飞, 史庆超, 孔令富, 毕保良, 宁丽军. TGF-β/Smads信号通路介导脯氨酸促进浅色黄姑鱼鱼鳔胶原蛋白沉积[J]. 水生生物学报, 2022, 46(11): 1649-1657. DOI: 10.7541/2022.2021.0275
引用本文: 荣华, 李德强, 马敏伟, 豆腾飞, 史庆超, 孔令富, 毕保良, 宁丽军. TGF-β/Smads信号通路介导脯氨酸促进浅色黄姑鱼鱼鳔胶原蛋白沉积[J]. 水生生物学报, 2022, 46(11): 1649-1657. DOI: 10.7541/2022.2021.0275
RONG Hua, LI De-Qiang, MA Min-Wei, DOU Teng-Fei, SHI Qing-Chao, KONG Ling-Fu, BI Bao-Liang, NING Li-Jun. TGF-Β/SMADS SIGNALING PATHWAY REGULATES PRO TO PROMOTE COLLAGEN DEPOSITION IN THE SWIM BLADDER OF CHU’S CROAKER (NIBEA COIBOR)[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1649-1657. DOI: 10.7541/2022.2021.0275
Citation: RONG Hua, LI De-Qiang, MA Min-Wei, DOU Teng-Fei, SHI Qing-Chao, KONG Ling-Fu, BI Bao-Liang, NING Li-Jun. TGF-Β/SMADS SIGNALING PATHWAY REGULATES PRO TO PROMOTE COLLAGEN DEPOSITION IN THE SWIM BLADDER OF CHU’S CROAKER (NIBEA COIBOR)[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1649-1657. DOI: 10.7541/2022.2021.0275

TGF-β/Smads信号通路介导脯氨酸促进浅色黄姑鱼鱼鳔胶原蛋白沉积

TGF-Β/SMADS SIGNALING PATHWAY REGULATES PRO TO PROMOTE COLLAGEN DEPOSITION IN THE SWIM BLADDER OF CHU’S CROAKER (NIBEA COIBOR)

  • 摘要: 为了进一步验证转化生长因子(TGF-β) /Smads信号通路在脯氨酸(Pro)促进鱼鳔胶原蛋白沉积中的作用, 研究利用TGF-β/Smads通路抑制剂干扰, 分析干扰前后鱼鳔中胶原蛋白含量及相关基因转录水平的变化。根据前期实验获得的Pro最佳添加量, 制定饲料配方, 饲喂浅色黄姑鱼1月后, 通过腹腔注射氧化苦参碱(Oxymatrine)为期1个月。在实验结束后, 测量各项指标, 评估干扰前后, Pro促进鱼鳔胶原蛋白沉积的效果及变化。实验结果表明: Pro能显著上调TGF-β/Smads通路中相关基因的表达, 并促进鱼鳔胶原蛋白沉积, Oxymatrine干扰显著下调了TGF-β/Smads通路中相关基因(Col1α1Col1α2TGF-βSmad2)表达, 同时导致鱼鳔中胶原蛋白沉积下降。由此推断Pro通过激活TGF-β/Smads通路促进鱼鳔中胶原蛋白沉积, 而Oxymatrine干扰抑制了TGF-β/Smads通路, 导致Smad2与Col1α2启动子结合受阻, 进而下调了Col1α2基因的转录, 从而降低鱼鳔中胶原蛋白的沉积。综上表明, TGF-β/Smads信号通路在Pro促进浅色黄姑鱼鱼鳔胶原蛋白沉积过程中起着重要调控作用, 其中Col1α1Col1α2TGF-βSmad2扮演着重要角色, 研究结果可为鱼类胶原蛋白代谢的营养调控提供新思路和新方法。

     

    Abstract: Swim bladder is an ideal optional source for collagen production and has long been considered as a traditional tonic in China. Therefore, strategies are required improve collagen content in the swim bladder for producing products with high nutrition and economic value. Proline (Pro) is the main proteinogenic amino acid needed for collagen production. Our previous study found that Pro can promote collagen deposition in the swim bladder of Chu’s croaker (Nibea coibor), and it is potentially regulated by TGF-β/Smad signaling pathway. In order to further verify the role of TGF-β/Smad signaling pathway in Pro promoting collagen deposition in swim bladder, the content collagen and transcription levels of related genes in the swim bladder of N. coibor were analyzed by inhibiting the TGF-β/Smad pathway. It was demonstrated that proline improved significantly the collagen content in the swim bladder of N. coibor, although no obvious enhancement in growth performance was observed. Proline supplementation up-regulated genes involved in collagen biosynthesis in the swim bladder. However, oxymatrine interference reduced significantly collagen deposition and the expression of Col1α1, Col1α2, TGF-β and Smad2 genes in the swim bladder of N. coibor. These results suggested that proline promoted collagen deposition in swim bladder mainly through regulating TGF-β/Smad signaling pathway, most likely via transient overexpression of Col1α1, Col1α2, TGF-β and Smad2 genes. Our results help to understand the molecular mechanism of collagen deposition in N. coibor for developing new strategies of producing fish products with higher quality.

     

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