Abstract: The issue of pulse pile-up is frequently encountered when the neutron is detected in intense radiation environment, which will distort the pulse shape and the neutron spectra. In this paper, a digital method of pulse pile-up identification and reconstruction was presented to solve the problem. The pulse pile-up was firstly identified by detecting the downward-going zero-crossings in the first-order derivative of the original signal, and then the constituent pulse was recovered based on four constructed models. The experiment results show that the method is capable of identifying pulse pile-up accurately, as well as disentangling two pulses with a minimum time interval of 20 ns, and it both improves neutrons’ effective counting rate and corrects the distorted neutron pulse spectrum due to pile-up event.