高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (7): 1612.doi: 10.7503/cjcu20121164

• 分析化学 • 上一篇    下一篇

基于AuNPs/PDDA-GO纳米复合物的电化学免疫传感器的构建及对SirT1蛋白的检测

毕文姬, 陆丹琴, 符莹, 黄齐林, 徐志爱, 张文   

  1. 华东师范大学化学系, 上海 200241
  • 收稿日期:2012-12-27 出版日期:2013-07-10 发布日期:2013-06-21
  • 通讯作者: 张 文, 男, 博士, 教授, 博士生导师, 主要从事生物电化学研究. E-mail: wzhang@chem.ecnu.edu.cn;徐志爱, 女, 博士, 副教授, 主要从事生物分析研究. E-mail: zaxu@chem.ecnu.edu.cn E-mail:wzhang@chem.ecnu.edu.cn;zaxu@chem.ecnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21075041)和高等学校博士学科点专项科研基金(批准号: 20110076110003)资助.

Electrochemical Immunosensor Based on AuNPs/PDDA-GO for Detection of SirT1

BI Wen-Ji, LU Dan-Qin, FU Ying, HUANG Qi-Lin, XU Zhi-Ai, ZHANG Wen   

  1. Department of Chemistry, East China Normal University, Shanghai 200241, China
  • Received:2012-12-27 Online:2013-07-10 Published:2013-06-21
  • Contact: wen zhang E-mail:wzhang@chem.ecnu.edu.cn;zaxu@chem.ecnu.edu.cn

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被引次数

3

摘要：

基于AuNPs/PDDA-GO纳米复合物制备了一种新型电化学免疫传感器, 并将其用于SirT1的检测. 首先, 在电极表面修饰复合材料AuNPs/PDDA-GO, 然后将目标蛋白SirT1固定到修饰了AuNPs/PDDA-GO的电极表面, 再通过特异性免疫反应结合一抗(Ab1)和辣根过氧化酶标记的二抗分子(HRP-Ab2), 最后用示差脉冲伏安法检测电流信号, 实现了对SirT1蛋白水平的测定. 在优化的实验条件下, SirT1蛋白的浓度在0.1~100 ng/mL范围内与响应电流呈良好线性关系, 检出限为0.029 ng/mL.

关键词: AuNPs/PDDA-GO纳米复合物, SirT1蛋白, 电化学免疫传感器

Abstract:

AuNPs/PDDA-GO nanocomposite was produced by combining poly(diallyldimethylammonium chloride)(PDDA) functionalized graphene oxide nanosheets(PDDA-GO) and gold nanoparticles(AuNPs) through self-assembly method, which offered an efficient platform for antibody immobilization with good biocompatibility. A new type of electrochemical immunosensor based on AuNPs/PDDA-GO nanocomposite for the detection of SirT1 was then developed. The immunosensor construction consisted of the immobilization of the hybrid architecture, the immunoreaction of target antigen SirT1 and capture antibody(Ab1), followed by the binding of HRP-Ab2. The designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range for SirT1 from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.029 ng/mL. Overall, this developed strategy is proved as a simple, cost-effective method, and can be easily extended to other protein analysis.

Key words: AuNPs/PDDA-GO nanocomposite, SirT1, Electrochemical immunosensor

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