目的 筛选和鉴定刺五加Eleutherococcus senticosus中的细胞色素P450基因（EsP450），分析其进化特征，探究其与刺五加总皂苷含量的相关性。方法 根据转录组测序结果，筛选得到EsP450基因，并对其进行生物信息学分析与适应性进化分析，采用qRT-PCR法检测EsP450基因的表达量，采用分光光度计测定刺五加的总皂苷含量。结果 筛选得到了18条EsP450基因。刺五加P450蛋白不存在跨膜区域，二级结构以α-螺旋和无规则卷曲结构为主。EsP450基因不存在正选择位点。各EsP450间的表达量差异显著，部分基因表达量差值在10倍以上，7条EsP450基因的表达与皂苷含量呈正相关关系（P<0.05）。结论 鉴定出与刺五加总皂苷含量存在正相关关系的EsP450基因，EsP450基因在进化中受到纯净选择。

Objective To screen and identify P450 gene in Eleutherococcus senticosus (EsP450), analyze its evolutionary characteristics, and explore the correlation between P450 gene and total saponins content of E. senticosus. Methods According to the results of transcriptome sequencing, EsP450 was screened and analyzed by bioinformatics and adaptive evolution, qRT-PCR was used to detect the expression of EsP450 and spectrophotometer was used to determine the total saponin content of E. senticosus. Results Eighteen EsP450 were screened. There was no transmembrane region in EsP450, and the secondary structure was mainly α-helix and irregular curly structure. There was no positive selection site of EsP450. The expression of EsP450 was significantly different. Seven EsP450 were identified, which were positively correlated with the total saponin content (P<0.05). Conclusion The EsP450 were subjected to purifying selection pressure during evolution.

R282.12

河北省自然科学基金生物医药联合基金项目（H2020209302）；河北省教育厅资助科研项目（ZD2019075）；国家自然科学基金项目（31570683）；河北省大学生创新创业训练计划（S202010081016）；华北理工大学大学生创新创业训练计划（R2020044）；河北省大中学生科技创新能力培育专项项目（2021H020911）