目的 基于不同浓度NaCl胁迫下黑果枸Lycium ruthenicum杞转录组测序结果，利用生物信息学方法对黑果枸杞bHLH转录因子家族成员进行鉴定。方法 通过对NaCl胁迫下黑果枸杞根和叶的样品进行转录组测序，筛选出bHLH家族基因，利用生物信息学方法对筛选到的基因进行理化性质、保守结构域、基因结构、细胞定位及系统进化分析。结果 黑果枸杞在NaCl胁迫下的转录组数据库中共筛选出89个bHLH转录因子家族基因；bHLH家族蛋白理化性质差异较大，71.90%的蛋白质呈弱酸性，蛋白属于亲水蛋白。黑果枸杞bHLH家族蛋白含有2个保守结构域，分别位于N端的碱性氨基酸区和C端的螺旋-环-螺旋区。亚细胞定位预测bHLH家族蛋白主要分布在细胞核内和细胞质中。进化分析显示，黑果枸杞中89个bHLH转录因子家族蛋白可分为20个亚族，其中第3亚族中包含的bHLH成员最多，为11个；第7、11、13、22亚族中的成员数量均为1个，其他亚族为2~9个。结论 NaCl胁迫下黑果枸杞bHLH转录因子家族有89个成员，bHLH家族蛋白大多数呈弱酸性，属亲水蛋白，可分为20个亚族。

Objective Based on the transcriptome sequencing results of Lycium barbarum under different concentrations of NaCl stress, the bHLH transcription factor family members of Lycium barbarum were identified by bioinformatics method. Methods The bHLH family genes were screened by transcriptome sequencing of leaf and root samples of Lycium ruthenicum Murr. under NaCl stress. Results The physicochemical properties, conserved domain, gene structure, cell location and phylogenetic development of these genes were analyzed by bioinformatics method. The results showed there were 89 bHLH family of Lycium ruthenicum. under NaCl stress. Their physicochemical properties were different, of which 71.90% proteins were weakly acidic and they were hydrophilic protein. The bHLH family of Lycium ruthenicum contained two conserved domains, which were located in the alkaline amino acid region of N and the helical ring and spiral region of C, respectively. The subcellular localization prediction of these bHLHs were mainly in the nucleus and extracellular. Phylogenetic analysis showed that 89 bHLH genes were divided into 20 subgroups, among which the 3 subgroup contained the most abundant bHLH members,including 11 bHLH proteins; the 7, 11, 13, 22 subgroups had only 1 member, respectively. In the other subgroups, the range was between 2 and 8. Conclusion There are 89 members of bHLH transcription factor family in Lycium barbarum under NaCl stress, and most of the bHLH family proteins are weakly acidic, belonging to hydrophilic proteins, which can be divided into 20 subgroups.

R286.12

国家自然科学基金资助项目（31760244）；国家自然科学基金资助项目（31560215）；甘肃农业大学科技创新项目（林学一级学科建设开放基金：GAU-XKJS-2018-112）；中国博士后科学基金资助项目（2017M613295XB）；甘肃农业大学青年研究生指导教师扶持基金项目（GAU-QNDS-201712）；甘肃省GEF/OP12三期项目专题研究项目（GS-GEF/OP12-02）