目的 研究大脑中动脉栓塞（MCAO）大鼠的脑纹状体代谢组学特征。方法 采用线栓栓塞大脑中动脉以复制MCAO大鼠模型，脑纹状体植入微透析探针，清醒、自由活动状态下采集脑微透析样品，正、负离子模式下超高效液相色谱-飞行时间质谱（UPLC-Q/TOF-MS）测定脑微透析样品，多元统计建立代谢组学模型。结果 成功进行了微透析技术的脑靶向性代谢组学研究，代谢组学模型可明显区分对照组和模型组，发现并鉴定了7个差异代谢物。结论 MCAO大鼠脑代谢特征发生了明显改变，基于微透析技术的代谢组学研究丰富了代谢组学的研究方法。

Objective To study the brain striatum metabolomics characteristics of middle cerebral artery occlusion (MCAO) rats. Methods The middle cerebral artery was occluded by monofilament to establish the MCAO rat model. The microdialysis probe was implanted into the striatum, and the brain microdialysis samples were collected under awake and free activity condition and were measured by UPLC-Q/TOF-MS in positive and negative ion mode respectively. Multivariate statistics were used to establish the metabobomics model. Results Brain-targeted metabolomics studies based on microdialysis samples were successfully performed. Metabolomics models could clearly distinguish normal and model rats, and seven differential metabolites were identified. Conclusion The brain metabolism characteristics of MCAO rats had changed significantly. The metabolomics research based on microdialysis technology enriches the research methods of metabolomics.

国家自然科学基金资助项目（81673889）；国家自然科学基金资助项目（81873227）；广东省科技计划项目（2014A020221102）