目的 测定姜黄饮片中姜黄素的含量，并建立姜黄的HPLC指纹图谱，评价⁶⁰Co-γ辐照对姜黄化学成分的影响。方法 对⁶⁰Co-γ辐照前后13批次姜黄饮片粉末中姜黄素含量进行测定；建立姜黄饮片HPLC指纹图谱并对其方法学进行考察。采用配对样本t检验和中药色谱指纹图谱相似度评价软件对辐照前后姜黄化学成分变化进行分析，结合聚类分析（HCA）和主成分分析（PCA）对辐照前、后样品进行综合评价，并对3批次姜黄样品辐照后3、6个月的稳定性进行了考察。结果 13批次姜黄粉末辐照前后姜黄素含量无统计学差异（P>0.05）；姜黄HPLC指纹图谱确定了16个共有峰，各批次姜黄辐照前后指纹图谱的相似度均大于0.998；同批次辐照前与辐照后样品聚类一致性较好，3批次辐照前后姜黄样品放置3、6个月后与0个月相比姜黄素含量变化RSD<5%，指纹图谱相似度均大于0.95。结论 ⁶⁰Co-γ辐照对姜黄中姜黄素含量和整体化学成分一致性基本无影响，且不影响其稳定性，通过指纹图谱结合指标成分含量分析可以更好地评价⁶⁰Co-γ辐照对姜黄化学成分的影响，为姜黄辐照灭菌方式的选择提供了实验依据。

Objective To evaluate the effect of irradiation of ⁶⁰Co-γ on composition change of Curcuma Longa through HPLC fingerprint analysis and determination of curcumin content. Methods An Agilent SB-C₁₈ column (250 mm×4.6 mm, 5 μm) was used as the column. Curcumin content was measured according to the method in 2015 edition of the “Pharmacopoeia of the People’s Republic of China”. Acetonitrile-0.1% phosphoric acid aqueous solution was used as the mobile phase of fingerprint analysis, and it was programmed in a gradient elution model and the detection wavelength was 240 nm. The effects of irradiation on content of curcumin and components change were analyzed by paired t-test and similarity evaluation. And then the comprehensive evaluation of constituent difference of C. Longa before and after irradiation was carried out by HCA and PCA analysis. Further more the stability of curcumin content and fingerprint similarity of three patches samples storage for 3 and 6 months was compared with that of 0 month. Results There was no significant difference before and after irradiation for curcumin content (P > 0.05). The fingerprints of C. Longae were established and 16 peaks were identified as the common peaks. The similarity of each batch before and after irradiation was more than 0.998 and the same batch before and after irradiation have better clustering consistency. The changes of curcumin content for 3 and 6 months compared with 0 month was less than 5%, and the fingerprint similarity was all greater than 0.95. Conclusion The established fingerprinting method is accurate and reliable. ⁶⁰Co-γ irradiation has no significant effect on the curcumin content and chemical composition consistency of C. Longa, which did not affect its stability. It was a good way to evaluate the ⁶⁰Co-γ irradiation effect on C. Longa by combining fingerprint analysis and the index components content determination. This method can provide reference for the utilization of irradiation on C. Longa.

江苏省医学重点人才项目（ZDRCA2016036）