目的 以大鼠胃溃疡模型为研究对象，研究中药复方戊己丸对大鼠胃溃疡治疗效应的谱效关系及药效物质基础。方法 在建立不同来源药材的11批戊己丸指纹图谱的基础上，采用大鼠胃溃疡模型，对11批戊己丸进行药效实验。运用灰色关联度分析法，将11批戊己丸药效数据与指纹图谱共有峰的相对峰面积相关联，建立谱效关系模型。结果 谱效关系研究结果表明，戊己丸对大鼠胃溃疡的药效作用是多种成分共同作用的结果，黄连中盐酸小檗碱、吴茱萸中吴茱萸碱、白芍中芍药苷等可显著降低胃组织中胃蛋白酶（PEP）的量，是戊己丸治疗胃溃疡的主要有效成分。结论 通过谱效关系研究获得了药效活性物质群，建立了戊己丸谱效关系评价的方法，客观反映药物内在质量，为该类中药制剂后期创新研究提供了重要的参考依据。

Objective To study the spectrum-effect relationship and material basis of pharmacodynamic effect of Wuji Pills in the treatment of gastric ulcer in rat model. Methods The analysis method for the fingerprint of 11 batches of Wuji Pills from different sources was built, and the rat model of gastric ulcer was used to investigate the pharmacodynamics of 11 batches of Wuji Pills. By system clustering method and gray relational analysis, efficacy data and relative peak areas of fingerprint common peaks of 11 batches of Wuji Pills were associated, and the related spectral efficiency relationship model was established. Results The spectrum-effect relationship showed that the therapeutic effect of Wuji Pills on rat gastric ulcer was a result of a variety of ingredients, the effect of berberine hydrochloride in Coptis Rhizoma, Evodia rutaecarpa alkali in Euodiae Fructus, paeoniflorin in Paeoniae Alba Radix, etc could significantly reduce the content of pepsin in stomach tissue (PEP), which were the main effective components in Wuji Pills. Conclusion The efficacy of active substances are obtained through the study on the relationship between spectrum and efficiency, establish the relationship between spectrum and efficiency evaluation of Wuji Pills, and the method provides important reference for the research of traditional Chinese medicine preparation.

国家自然科学基金资助项目（81460609）