目的 整合UHPLC-Q Exactive轨道肼高分辨质谱与HPLC-CL技术分析酸枣叶中主要化学成分，追踪其抗氧化活性成分。方法 采用UHPLC-Q Exactive轨道肼高分辨质谱鉴定酸枣叶中化学成分；并建立不同产地酸枣叶样品HPLC-UV指纹图谱；利用HPLC-CL方法对8个不同产地的酸枣叶指纹图谱进行抗氧化活性评价。结果 酸枣叶中共鉴定10个化合物，均为黄酮苷类化合物，8批不同产地的酸枣叶相似度均大于0.9，并最终确定其中6种物质（eriodictyol-7-O-rutinoside、quercetin-3-O-robinobioside、quercetin-3-O-rutinoside、quercetin-3-O-β-D-galactoside、quercetin-3-O-β-D-glucoside、quercetin-3-O-β-L-arabinosyl-（1-2）-α-L-rhamnoside）清除自由基能力强。结论 酸枣叶中富含大量的黄酮类成分，且具有较好的抗氧化活性。酸枣叶可为市场提供一种新型绿色的、可持续的抗氧化剂来源。

Objective To integrate UHPLC-Q Exactive Orbitrap-HRMS and HPLC-CL to study the major chemical constituents in Ziziphi Spinosae Folium (the leaves of Ziziphus jujuba var. spinosa) and to trace the active constituents on line. Methods A UHPLC-Q Exactive Orbitrap-HRMS method was developed for the identification of multi-constituents in Ziziphi Spinosae Folium. The fingerprint for the multi-component assay in the leaves was established by HPLC-UV. The biological activity fingerprint of Ziziphi Spinosae Folium scavenging H₂O₂ and O₂^·- was simultaneously identified for eight different areas in Shanxi. Results Ten compounds in the leaves were identified or tentatively characterized. Among them, six flavones have scavenging activities for free radicals. Thirteen common peaks were calibrated and the correlation coefficients were greater than 0.90. Conclusion A large number of flavones in Ziziphi Spinosae Folium have antioxidant activities. Ziziphi Spinosae Folium provides a novel, green, and sustainable source as antioxidant for the market.

山西省卫生厅青年科技攻关项目（20100124）；山西省科技厅青年基础研究计划项目（2012021031-5）