目的 对祁州漏芦Rhaponticum uniflorum的花序进行化学成分研究。方法 利用大孔树脂、硅胶、MCI、Sephadex LH-20等柱色谱技术分离纯化，根据理化性质和波谱数据鉴定化合物的结构。结果 从漏芦花乙醇提取物中分离鉴定了17个化合物，其中11个黄酮类：5,7,4'-三羟基-3'-甲氧基黄酮（1）、槲皮素-3'-O-甲醚（2）、芹菜素（3）、山柰酚（4）、木犀草素（5）、槲皮素（6）、芹菜素-7-O-β-D-葡萄糖醛酸乙酯（7）、山柰酚-3-O-α-L-鼠李糖苷（8）、槲皮素-3-O-α-L-鼠李糖苷（9）、芹菜素-7-O-β-D-葡萄糖醛酸苷（10）、芹菜素-6,8-二-C-β-D-葡萄糖苷（11）；2个木脂素类：泥胡木脂素B苷（12）、泥胡木脂素B（13）；2个植物蜕皮激素类：蜕皮甾酮（14）和土克甾酮（15）；其余2个为熊果酸（16）和3,5-O-二咖啡酰基-奎宁酸（17）。结论 化合物1、2、7～10、12、13为首次从漏芦属植物中分离得到，化合物1、2、7～17为首次从祁州漏芦花序中分离得到。

Objective To investigate the chemical constituents from the flowers of Rhaponticum uniflorum. Methods The chemical constituents were separated and purified by macroporous resin, silica gel, Sephadex LH-20, and MCI column chromatographies. Their structures were determined by physicochemical properties and spectral data. Results Seventeen compounds were isolated from the ethanol extract in the flowers of R. uniflorum. Among them, eleven flavones were identified as 5,7,4'-trihydroxy-3'-methoxyflavone (1), quercetin-3'-O-methyl ether (2), apigenin (3), kaempferol (4), luteolin (5), quercetin (6), apigenin-7-O-β-D-glycuronate ethyl ester (7), kaempferol-3-O-α-L-rhamnoside (8), quercetin-3-O-α-L-rhamnoside (9), apigenin-7-O-β-D-glucopyranoside (10), and apigenin-6,8-di-C-β-D-glucoside (11); Two liganans were hemislin B glucoside (12) and hemislin B (13); Two phytoecdysones were ecdysterone (14) and turkesterone (15); Others were ursolic acid (16) and 3,5-O-dicaffeoyl quinic acid (17). Conclusion Compounds 1, 2, 7—10, 12 and 13 are isolated from the plants of Rhaponticum Cass. for the first time, and the compounds 1, 2, and 7—17 are found from the flowers of R. uniflorum for the first time.

内蒙古自治区科技创新引导项目（2015cztxyd02）