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Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ruidong Xiang conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Victor Hutton Oddy wrote the paper, reviewed drafts of the paper, contribution of interpretation of results.

Alan L. Archibald analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, contribution of interpretation of results.

Phillip E. Vercoe wrote the paper, reviewed drafts of the paper, contribution of interpretation of results.

Brian P. Dalrymple conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The research in this article used published datasets (European Nucleotide Archive study accession PRJEB6169) and did not generate any raw data. The major secondary datasets are included in the supplementary material.

Funding

This work was supported by the Department of Agriculture, Filling the Research Gap “International Coordination of the Rumen Pangenome project” FTRG-1194147-75. ALA and RT acknowledge support from BBSRC Institute Strategic Programme Grants. The Ensembl annotation was funded by BBSRC BB/I025328/1. Sequencing was carried out by Edinburgh Genomics, The University of Edinburgh. Edinburgh Genomics is partly supported through core grants from NERC (R8/H10/56), MRC (MR/K001744/1) and BBSRC (BB/J004243/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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