TMC-205 a New Transcriptional Up-Regulator of SV40 Promoter Produced by an Unidentified Fungus Fermentation, Isolation, Physico-chemical Properties, Structure Determination and Biological Activities

MASAAKI SAKURAI; JUN KOHNO; MAKI NISHIO; KOZO YAMAMOTO; TORU OKUDA; KIMIO KAWANO; NORIYUKI NAKANISHI

doi:10.7164/antibiotics.54.628

TMC-205 a New Transcriptional Up-Regulator of SV40 Promoter Produced by an Unidentified Fungus

Fermentation, Isolation, Physico-chemical Properties, Structure Determination and Biological Activities

MASAAKI SAKURAI, JUN KOHNO, MAKI NISHIO, KOZO YAMAMOTO, TORU OKUDA, KIMIO KAWANO, NORIYUKI NAKANISHI

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JOURNAL FREE ACCESS

2001 Volume 54 Issue 8 Pages 628-634

DOI https://doi.org/10.7164/antibiotics.54.628

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Abstract

A new transcriptional up-regulator designated TMC-205 was discovered from the fermentation broth of an unidentified fungal strain TC 1630 by using an SV40 promoterluciferase reporter assay. Based on spectroscopic analyses, its structure was determined to be (E)-6-(3-methyl-1, 3-butadienyl)-1H-indole-3-carboxylic acid. Expression of the luciferase activity was activated ca. 2-, 4-, and 6-fold by 1, 10, and 100μM TMC-205, respectively. TMC-205 activated the transcriptional activity in a manner dependent on the presence of the enhancer element of SV40 in its promoter region.

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