Amphistin, a New Melanogenesis Inhibitor, Produced by an Actinomycete

NORIKO ARAI; KAZURO SHIOMI; SATOSHI TAKAMATSU; KANKI KOMIYAMA; MAYUMI SHINOSE; YOKO TAKAHASHI; YOSHITAKE TANAKA; YUZURU IWAI; JING-RONG LIU; SATOSHI OMURA

doi:10.7164/antibiotics.50.808

NORIKO ARAI, KAZURO SHIOMI, SATOSHI TAKAMATSU, KANKI KOMIYAMA, MAYUMI SHINOSE, YOKO TAKAHASHI, YOSHITAKE TANAKA, YUZURU IWAI, JING-RONG LIU, SATOSHI OMURA

Author information

NORIKO ARAI
Research Center for Biological Function, The Kitasato Institute
KAZURO SHIOMI
Research Center for Biological Function, The Kitasato Institute
SATOSHI TAKAMATSU
Research Center for Biological Function, The Kitasato Institute
KANKI KOMIYAMA
Research Center for Biological Function, The Kitasato Institute
MAYUMI SHINOSE
Research Center for Biological Function, The Kitasato Institute
YOKO TAKAHASHI
Research Center for Biological Function, The Kitasato Institute
YOSHITAKE TANAKA
Research Center for Biological Function, The Kitasato Institute
YUZURU IWAI
Research Center for Biological Function, The Kitasato Institute
JING-RONG LIU
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences
SATOSHI OMURA
Research Center for Biological Function, The Kitasato Institute

JOURNAL FREE ACCESS

1997 Volume 50 Issue 10 Pages 808-814

DOI https://doi.org/10.7164/antibiotics.50.808

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Abstract

A new melanogenesis inhibitor, named amphistin, was isolated from the fermentation broth of an actinomycete strain KP-3052. Amphistin was purified from the culture filtrate by the combination of cation exchange, gel filtration, and aminosilyl silica gel chromatographic methods. The structure of amphistin was elucidated as γ-(β-histidinoalanino)homoalanine by NMR experiments including ¹H-¹⁵N HMBC experiment and other spectroscopic analyses. Amphistin inhibited the melanogenesis of B16 melanoma cells at concentration of 6.8μM.

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