ISOLATION AND CHARACTERIZATION OF NEW THIOL PROTEASE INHIBITORS ESTATINS A AND B

SATOSHI YAGINUMA; AKIRA ASAHI; ATSUKI MORISHITA; MITSUO HAYASHI; MASATOSHI TSUJINO; MASAKI TAKADA

doi:10.7164/antibiotics.42.1362

SATOSHI YAGINUMA, AKIRA ASAHI, ATSUKI MORISHITA, MITSUO HAYASHI, MASATOSHI TSUJINO, MASAKI TAKADA

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JOURNAL FREE ACCESS

1989 Volume 42 Issue 9 Pages 1362-1369

DOI https://doi.org/10.7164/antibiotics.42.1362

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Abstract

New thiol protease inhibitors, estatins A and B, were isolated from the culture filtrate of Myceliophthora thermophila M4323. The basic, water-soluble inhibitors were characterized as having an agmatine, tuans-epoxysuccinic acid and L-phenylalanine or L-tyrosine moieties in the structure. The molecular formulas C₁₈H₂₅N₅O₅ and C₁₈H₂₅N₅O₆ for A and B were indicated by elemental analysis and fast atom bombardment MS. Estatins were specific inhibitors against thiol proteases such as papain, ficin and bromelain. They suppressed IgE antibody production in mice, but not IgG.

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