The purpose of this study was to explore the relationships among personality traits, organizational commitment, and flow experience among female healthcare volunteers working in Changhua, County, Taiwan. After adopting a convenient sampling method, the survey questionnaires were distributed to 522 female healthcare volunteers, yielding 397 valid responses. The findings showed a volunteer profile of married housewives with three children, having a high-school diploma, a mean age of 44.47 years and Taoism as their religious faith; devoting 3.38 years and an average of 18.71 hours per month to voluntary work for an organization; having ”self-growth” as the volunteerism motive and rating training received ns ”satisfied.” The bivariate and multivariate analyses revealed: (1) The number of organizations served and satisfaction with training were positively related to flow experience and organizational commitment. (2) The volunteers with a salient Type A personality tended to have higher ”self-efficacy” and ”self-control” of flow; conversely, individuals with Type B traits showed a higher degree of ”continuance” and ”effort” in organizational commitment. (3) ”Self-efficacy,” ”service as autotelic experience,” ”concentration on the task,” ”loss of self-consciousness,” and ”self-control” of flow were positively correlated with ”goal/value” and ”effort” of organizational commitment. (4) Personality traits, organizational commitment, and satisfaction with training manifested significant predictive power over flow experience. Female volunteers with stronger Type A orientation, higher overall organizational commitment and higher satisfaction with training tended to experience higher flow. Furthermore, personality trait held the greatest predictive power over overall flow experience. Finally, on the basis of the results, discussions are presented and recommendations for future research in public and non-profit organizations are made.