This research aims to understand the correlation between comprehensive vocation subject’s teachers’ emotional labor and job burnout and the differentiation with different background variables on those. The questionnaire method was used, comprehensive vocation subject’s teachers at senior vocational schools who have been teaching in school year 2009 to 2010 were chosen as participants and “emotional labor and job burnout” questionnaire was developed as the instrument in this study. 446 questionnaires were distributed and 369 effective questionnaires which accounted for 82.6%. Descriptive statistics, one-sample t-test, independent t-test, one-way ANOVA, and canonical correlation were adopted and the conclusions were as follows: 1. Teacher’s emotional labor performed over upper-intermediate level, teachers’ job burnout performed over intermediate level. 2. Teacher’s emotional labor verified by their age, position they stayed the longest, marriage, years of service, and special education qualification. 3. Teacher’s labor burnout verified by marriage. 4.When teachers had higher feelings of emotional labor, they would have lower feelings of job burnout which includes two dimensions, depersonalization and lack of person accomplishment. Based on the results, the suggestions are provided for education administration, teachers, and follow-up researches as a reference.