With the prevalence of artificial lighting and the change of life style, most people spend 80% to 90% of their time staying indoors, the stimulus of artificial lighting brings modern people physiological and psychological effects which have been becoming one of the most important issues. Scholars have been long concerned about the conditions to supply a suitable working and learning environment with appropriate lighting. Since the recent studies of color temperature and psychological evaluation has some flaws which hardly match practical situation, this research will, on the contrary, be set with a real situation – under different color temperatures, what effects will they cause long-time workers early, middle, and late psychological evaluation? This research aims to find out the subject evaluation by basically studying the testers’ visual and reading behaviors, under 7801x, 150 lx illumination, and five-color-temperature condition. The results of this study show that,(1) in those five color temperature conditions, people’s sensation of comfort and glare, tension, stress degree, tiredness and the entire satisfaction degree are not significantly affected by the color temperature but the time. (2) Under 780 lx-3000K and 1501x – 6500K, testers feel relaxed and comfortable. (3) Under 7801x – 6500K, people’s recognition rate of gray pattern in five lighting conditions is the highest. (4) Illumination level 7801x can make testers more comfortable when they have engaged in visual activities for long, except for the effects of color temperature.