Accident-induced craniomaxillofacial injury frequently results in large bony defects which require the bone grafts to enhance the bone healing. Biodegradable polymer [Poly(L-lactide), PLLA] scaffolds are the devices which are popular used in the reconstruction of craniomaxillofacial defects because of their good biocompatibility、biodegradability and low toxicity after degradation in vivo. However, low proliferation rate and poor cell attachment were found when culturing cell on the PLLA membrane. So many geometrical and chemical strategies were undertaken to improve cells growth on the PLLA surface. In this study, static magnetic fields were non-invasively applied to our cell cultured system to improve the osteoblast-like cells growth on the PLLA membrane. Cellular models (MG63 and MC3T3-E1, osteoblast-like cells) were carried out to examine the hypothesis that static magnetic fields (SMF) affect osteoblast-like cells growth on the PLLA membrane. The cells were continuously exposed to 4000 Gauss-SMF for 5 days. The proliferation effects of SMF were tested by MTT assay. The morphologic changes and extracellular matrix release were observed by the scanning electron microscopy. In addition, the effects of SMF on the alkaline phosphatase activity levels were compared between the exposed and unexposed cells. Scanning electron microscopy studies indicated that SMF promoted MG63 cells and MC3T3-E1 cells express extracellular matrix on the PLLA membrane. The SMF exposed MG63 cells exhibited decreased MTT values at 2 and 3 days of culture. Besides, the SMF exposed MC3T3-E1 cells exhibited increased MTT values at 3 and 4 days of culture. At 1 day of observation, the alkaline phosphatase-specific activity of the SMF exposed MG63 cells demonstrated a maximum level and significantly larger than that of the unexposed cells (p<0.05) with an increasing ratio of 1.5 folds. The SMF exposed MC3T3-E1 cells express the lower alkaline phosphatase-specific activity than that of the unexposed cells at 3 and 4 days of culture (p<0.05). These results reveal that MG63 cells, seeding on the PLLA membrane, treated with SMF exhibit a more differentiated morphology and express a more differentiated regulatory marker. Furthermore, SMF can promote MC3T3-E1 cells growth on the PLLA membrane.