Objective: The purpose of the study was to investigate the efficacy of twelve weeks modified Pilates training on low back pain patients. Methods: All subjects undertook the modified Pilates training two times (sixty minutes per time) a week during three months. They underwent the following measurements: height (RT), weight (WT), Oswestry Low Back Pain Disability Questionnaire, visual analog scale (VAS). The data were analyzed by descriptive statistics and two-way ANOVA with a=0.05 significant level to examine any changes after the training. Results: Results showed significant improvements in daily life dysfunction (11.7 ± 7.1 vs. 8.1 ± 6.2) and pain severity (6.5 ± 1.5 vs. 2.9 ± 1.4) but no statistically significant changes between two groups on sleeping quality. Conclusions: Twelve weeks modified Pilates training was effective in both groups with reduced the disability of daily life and decreased pain severity.